VIP21/caveolin is a Cholesterol-binding Protein

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1995 Oct 24

PMID 7479780

Citations 258

Authors

M Murata

J Peranen

R Schreiner

F Wieland

T V Kurzchalia

K Simons

Affiliations

Soon will be listed here.

Abstract

VIP21/caveolin is localized to both caveolae and apical transport vesicles and presumably cycles between the cell surface and the Golgi complex. We have studied the lipid interactions of this protein by reconstituting Escherichia coli-expressed VIP21/caveolin into liposomes. Surprisingly, the protein reconstituted only with cholesterol-containing lipid mixtures. We demonstrated that the protein binds at least 1 mol of cholesterol per mole of protein and that this binding promotes formation of protein oligomers. These findings suggest that VIP21/caveolin, through its cholesterol-binding properties, serves a specific function in microdomain formation during membrane trafficking.

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References

Rothberg K, Ying Y, Kamen B, Anderson R . Cholesterol controls the clustering of the glycophospholipid-anchored membrane receptor for 5-methyltetrahydrofolate. J Cell Biol. 1990; 111(6 Pt 2):2931-8. PMC: 2116385. DOI: 10.1083/jcb.111.6.2931. View

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View

Anderson R, Kamen B, Rothberg K, Lacey S . Potocytosis: sequestration and transport of small molecules by caveolae. Science. 1992; 255(5043):410-1. DOI: 10.1126/science.1310359. View

Brown D, Rose J . Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell. 1992; 68(3):533-44. DOI: 10.1016/0092-8674(92)90189-j. View

Simionescu N, Siminoescu M, Palade G . Permeability of muscle capillaries to small heme-peptides. Evidence for the existence of patent transendothelial channels. J Cell Biol. 1975; 64(3):586-607. PMC: 2109550. DOI: 10.1083/jcb.64.3.586. View

Montesano R, Roth J, Robert A, Orci L . Non-coated membrane invaginations are involved in binding and internalization of cholera and tetanus toxins. Nature. 1982; 296(5858):651-3. DOI: 10.1038/296651a0. View

Schroeder F, DEMPSEY M, Fischer R . Sterol and squalene carrier protein interactions with fluorescent delta 5,7,9(11)-cholestatrien-3 beta-ol. J Biol Chem. 1985; 260(5):2904-11. View

Tran D, Carpentier J, Sawano F, Gorden P, Orci L . Ligands internalized through coated or noncoated invaginations follow a common intracellular pathway. Proc Natl Acad Sci U S A. 1987; 84(22):7957-61. PMC: 299455. DOI: 10.1073/pnas.84.22.7957. View

Glenney Jr J . Tyrosine phosphorylation of a 22-kDa protein is correlated with transformation by Rous sarcoma virus. J Biol Chem. 1989; 264(34):20163-6. View

10.

Rothberg K, Heuser J, Donzell W, Ying Y, Glenney J, Anderson R . Caveolin, a protein component of caveolae membrane coats. Cell. 1992; 68(4):673-82. DOI: 10.1016/0092-8674(92)90143-z. View

11.

Chang W, Rothberg K, Kamen B, Anderson R . Lowering the cholesterol content of MA104 cells inhibits receptor-mediated transport of folate. J Cell Biol. 1992; 118(1):63-9. PMC: 2289528. DOI: 10.1083/jcb.118.1.63. View

12.

Kurzchalia T, Dupree P, Parton R, Kellner R, Virta H, Lehnert M . VIP21, a 21-kD membrane protein is an integral component of trans-Golgi-network-derived transport vesicles. J Cell Biol. 1992; 118(5):1003-14. PMC: 2289580. DOI: 10.1083/jcb.118.5.1003. View

13.

Glenney Jr J . The sequence of human caveolin reveals identity with VIP21, a component of transport vesicles. FEBS Lett. 1992; 314(1):45-8. DOI: 10.1016/0014-5793(92)81458-x. View

14.

Dupree P, Parton R, Raposo G, Kurzchalia T, Simons K . Caveolae and sorting in the trans-Golgi network of epithelial cells. EMBO J. 1993; 12(4):1597-605. PMC: 413373. DOI: 10.1002/j.1460-2075.1993.tb05804.x. View

15.

Fiedler K, Kobayashi T, Kurzchalia T, Simons K . Glycosphingolipid-enriched, detergent-insoluble complexes in protein sorting in epithelial cells. Biochemistry. 1993; 32(25):6365-73. DOI: 10.1021/bi00076a009. View

16.

Ying Y, Anderson R, Rothberg K . Each caveola contains multiple glycosyl-phosphatidylinositol-anchored membrane proteins. Cold Spring Harb Symp Quant Biol. 1992; 57:593-604. DOI: 10.1101/sqb.1992.057.01.065. View

17.

Parton R . Ultrastructural localization of gangliosides; GM1 is concentrated in caveolae. J Histochem Cytochem. 1994; 42(2):155-66. DOI: 10.1177/42.2.8288861. View

18.

Wang X, Sato R, Brown M, Hua X, Goldstein J . SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis. Cell. 1994; 77(1):53-62. DOI: 10.1016/0092-8674(94)90234-8. View

19.

Trotter P, Voelker D . Lipid transport processes in eukaryotic cells. Biochim Biophys Acta. 1994; 1213(3):241-62. DOI: 10.1016/0005-2760(94)00073-5. View

20.

Smart E, Ying Y, Conrad P, Anderson R . Caveolin moves from caveolae to the Golgi apparatus in response to cholesterol oxidation. J Cell Biol. 1994; 127(5):1185-97. PMC: 2120264. DOI: 10.1083/jcb.127.5.1185. View