Multiple MRNA Species for Adenovirus Type 2 Polypeptides III and PVII

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1980 Aug 1

PMID 7441815

Citations 3

Authors

C B Lawrence

Affiliations

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Abstract

Fractionation of messenger activities isolated from the cytoplasm of HeLa cells late in infection with adenovirus type 2 reveals that viral polypeptides III and pVII are each synthesized from two different-sized mRNA's. the major messenger activity for each protein has the same sedimentation rate as that previously reported by Anderson et al. (Proc. Natl. Acad. Sci. U.S.A. 71:2756-2760, 1974). The minor messenger activities for III and pVII sediment more rapidly and are not aggregates of the major mRNA's for these proteins. The two minor messenger activities cosediment with two polyadenylated RNA species which are labeled late in infection with 32P and whose molecular weights are estimated to be 2.9 x 10(6) and 2.4 x 10(6). Both of these species hybridize to adenovirus type 2 DNA specific for the mRNA family that is 3' coterminal at adenovirus type 2 map position 49.5 and the mRNA family that is 3' coterminal at 62.0. This is consistent with the possibility that these RNAs have 5'-terminal sequences identical to those of the normal mRNA's for III and pVII but are 3' coterminal at map position 62, the normal 3' terminus of the mRNA's for polypeptides II and pVI. These species are not found in polyadenylated RNA isolated from the nucleus, suggesting that the minor mRNA species are cytoplasmic RNAs.

Citing Articles

Adenovirus type 2 fiber mRNA synthesis: no evidence for a cytoplasmic processing pathway.

Lawrence C, Ramsey W J Virol. 1982; 44(1):226-34.

PMID: 6292486 PMC: 256256. DOI: 10.1128/JVI.44.1.226-234.1982.

Physical mapping of adenovirus type 2 temperature-sensitive mutations by restriction endonuclease analysis of interserotypic recombinants.

DHalluin J, Cousin C, BOULANGER P J Virol. 1982; 41(2):401-13.

PMID: 6281456 PMC: 256770. DOI: 10.1128/JVI.41.2.401-413.1982.

Expression of adenovirus type 2 late genes in HeLa cells infected with adenovirus type 2 or adenovirus type 2-simian virus 40 hybrid viruses.

Lawrence C J Virol. 1981; 40(2):610-4.

PMID: 6275108 PMC: 256667. DOI: 10.1128/JVI.40.2.610-614.1981.

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