Physical Mapping of Adenovirus Type 2 Temperature-sensitive Mutations by Restriction Endonuclease Analysis of Interserotypic Recombinants

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1982 Feb 1

PMID 6281456

Citations 5

Authors

J C DHalluin

C Cousin

P BOULANGER

Affiliations

Soon will be listed here.

Abstract

The genome structures of about 100 interserotypic ts recombinants produced in crosses between human adenovirus type 2 (H2) and 5 (H5) temperature-sensitive mutants were analyzed by cleavage with restriction endonucleases to determine the map coordinates of the following temperature-sensitive mutants: penton base plus fiber-defective H2 ts103, -104, and -136, assembly-defective H2 ts112, fiber-defective H2 ts125, hexon-defective H2 ts118 and -121, and DNA-negative H2 ts111. H5 ts1 (100 K defective), H5 ts36 (DNA negative), H5 ts125 (mutated in the early 72,000-dalton protein), H5 ts22 (fiber defective), H5 ts58 (IIIa defective), and H5 ts18 and -19 were used as one of the parents. The physical locations of the H2 temperature-sensitive mutations thus defined are discussed in relation to the genetic map, the biological function altered, and the positions of the structural genes on the genome.

Citing Articles

Structural and functional determinants in adenovirus type 2 penton base recombinant protein.

Karayan L, Hong S, Gay B, TOURNIER J, DAngeac A, BOULANGER P J Virol. 1997; 71(11):8678-89.

PMID: 9343226 PMC: 192332. DOI: 10.1128/JVI.71.11.8678-8689.1997.

Biochemical and genetical characterization of a fiber-defective temperature-sensitive mutant of type 2 adenovirus.

Boudin M, Rigolet M, Lemay P, Galibert F, BOULANGER P EMBO J. 1983; 2(11):1921-7.

PMID: 6571701 PMC: 555389. DOI: 10.1002/j.1460-2075.1983.tb01680.x.

Independent mutations in Ad2ts111 cause degradation of cellular DNA and defective viral DNA replication.

Stillman B, White E, Grodzicker T J Virol. 1984; 50(2):598-605.

PMID: 6368865 PMC: 255680. DOI: 10.1128/JVI.50.2.598-605.1984.

Isolation and characterization of temperature-sensitive mutants of adenovirus type 7.

Praszkier J, Ginsberg H J Virol. 1987; 61(10):3089-95.

PMID: 3041031 PMC: 255884. DOI: 10.1128/JVI.61.10.3089-3095.1987.

Processing of vimentin occurs during the early stages of adenovirus infection.

Belin M, BOULANGER P J Virol. 1987; 61(8):2559-66.

PMID: 3037116 PMC: 255697. DOI: 10.1128/JVI.61.8.2559-2566.1987.

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