Effect of Environmental PH on Chain Length of Lactobacillus Bulgaricus

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1980 Dec 1

PMID 7440507

Citations 6

Authors

S K Rhee

M Y Pack

Affiliations

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Abstract

Culture medium pH was found to affect strongly the chain length of lactobacillus bulgaricus NLS-4 cells. The organism was cultured continuously in glucose-limited complex medium of different pH's with constant agitation at 250 rpm under anaerobic headspace. The cell chains increased their lengths with an increase in pH and yielded clumps of folded filaments at pH above 8.0. Involvement of an autolytic enzyme(s) in the separation of L. bulgaricus cells was confirmed, and the poor synthesis of this enzyme(s) under alkaline culture conditions could explain the pH-related filamentous growth of this organism.

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References

Rhee S, Pack M . Effect of environmental pH on fermentation balance of Lactobacillus bulgaricus. J Bacteriol. 1980; 144(1):217-21. PMC: 294626. DOI: 10.1128/jb.144.1.217-221.1980. View

LOMINSKI I, Cameron J, WYLLIE G . Chaining and unchaining Streptococcus faecalis; a hypothesis of the mechanism of bacterial cell separation. Nature. 1958; 181(4621):1477. DOI: 10.1038/1811477a0. View

EKSTEDT R, STOLLERMAN G . Factors affecting the chain length of group A streptococci. I. Demonstration of a metabolically active chain-splitting system. J Exp Med. 1960; 112:671-86. PMC: 2137239. DOI: 10.1084/jem.112.4.671. View

Young F, Spizizen J . BIOCHEMICAL ASPECTS OF COMPETENCE IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM. II. AUTOLYTIC ENZYME ACTIVITY OF CELL WALLS. J Biol Chem. 1963; 238:3126-30. View

de Vries W, Kapteijn W, van der Beek E, Stouthamer A . Molar growth yields and fermentation balances of Lactobacillus casei L3 in batch cultures and in continuous cultures. J Gen Microbiol. 1970; 63(3):333-45. DOI: 10.1099/00221287-63-3-333. View