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» Articles » PMID: 4988245

Cell Wall Binding Properties of the Bacillus Subtilis Autolysin(s)

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1970 Aug 1
PMID 4988245
Citations 25
Authors
D P Fan
Affiliations
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Abstract

Cell walls isolated from exponentially growing Bacillus subtilis have autolysin(s) attached to them. An autolysin can be released from the walls by incubation at 0 C with 3 m LiCl. The enzyme can reattach to walls when the salt concentration is reduced. The bound enzyme cannot be removed or destroyed by washing the walls with 8 m urea at 0 C. The binding of free enzyme to walls at 0 C can take place normally in the presence of 2 m urea.

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Autolysin(s) of Bacillus subtilis as dechaining enzyme.

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References
1.
Young F . Autolytic enzyme associated with cell walls of Bacillus subtilis. J Biol Chem. 1966; 241(15):3462-7. View
2.
SHOCKMAN G, Cheney M . Autolytic enzyme system of Streptococcus faecalis. V. Nature of the autolysin-cell wall complex and its relationship to properties of the autolytic enzyme of Streptococcus faecalis. J Bacteriol. 1969; 98(3):1199-207. PMC: 315314. DOI: 10.1128/jb.98.3.1199-1207.1969. View
3.
Stewart C, MARMUR J . Increase in lytic activity in competent cells of Bacillus subtilis after uptake of deoxyribonucleic acid. J Bacteriol. 1970; 101(2):449-55. PMC: 284927. DOI: 10.1128/jb.101.2.449-455.1970. View
4.
Brown W, Young F . Dynamic interactions between cell wall polymers, extracellular proteases and autolytic enzymes. Biochem Biophys Res Commun. 1970; 38(4):564-8. DOI: 10.1016/0006-291x(70)90618-2. View
5.
Fan D . Autolysin(s) of Bacillus subtilis as dechaining enzyme. J Bacteriol. 1970; 103(2):494-9. PMC: 248109. DOI: 10.1128/jb.103.2.494-499.1970. View