New Deletion Mutant of Bacteriophage T5

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1980 Nov 1

PMID 7431490

Citations 2

Authors

M Rhoades

J Schwartz

J M Wahl

Affiliations

Soon will be listed here.

Abstract

Deletion mutants of bacteriphage T5 can be identified by analyzing the DNA content of single plaques, after alkaline denaturation, by agarose gel electrophoresis. Use of this procedure has resulted in the isolation of a new mutant, designated del2, that lacks a 1.6% segment located 68 to 70% from the left end of T5 DNA. This segment occurs between the early and late gene regions of T5 DNA.

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Islands of non-essential genes, including a DNA translocation operon, in the genome of bacteriophage 0305ϕ8-36.

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New physical map of bacteriophage T5 DNA.

Rhoades M J Virol. 1982; 43(2):566-73.

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