Bacteriophage T5-induced Endonucleases That Introduce Site-specific Single-chain Interruptions in Duplex DNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 May 1

PMID 5725

Citations 15

Authors

S G Rogers

M Rhoades

Affiliations

Soon will be listed here.

Abstract

Four site-specific endodeoxyribonucleases have been partially purified from extracts of bacteriophage T5-infected Escherichia coli by gel filtration and affinity chromatography on single- and double-stranded DNA. The enzymes were detected and characterized by agarose gel electrophoresis of alkali-denatured digestion products. None of the four is found in uninfected cells. In the presence of a divalent cation, all four endonucleases make ligase-repairable, single-chain interruptions at specific sites in the duplex DNA of several bacteriophages (lambda, T7, and T5) and a mammalian virus (adenovirus 2). These activities are not stimulated by ATP. None of the four is active on single-stranded DNA. The fragments produced by each enzyme from ligase-repaired T5 DNA do not correspond to those derived from mature T5 DNA. Each of the enzymes is able to cleave the intact strand of T5 DNA.

Citing Articles

Structure of the herpes simplex virus 1 genome: manipulation of nicks and gaps can abrogate infectivity and alter the cellular DNA damage response.

Smith S, Reuven N, Mohni K, Schumacher A, Weller S J Virol. 2014; 88(17):10146-56.

PMID: 24965466 PMC: 4136335. DOI: 10.1128/JVI.01723-14.

Identification of host receptor and receptor-binding module of a newly sequenced T5-like phage EPS7.

Hong J, Kim K, Heu S, Lee S, Adhya S, Ryu S FEMS Microbiol Lett. 2008; 289(2):202-9.

PMID: 19025561 PMC: 9021807. DOI: 10.1111/j.1574-6968.2008.01397.x.

Structure-specific DNA binding by bacteriophage T5 5'-->3' exonuclease.

Garforth S, Sayers J Nucleic Acids Res. 1997; 25(19):3801-7.

PMID: 9380501 PMC: 146983. DOI: 10.1093/nar/25.19.3801.

New deletion mutant of bacteriophage T5.

Rhoades M, Schwartz J, Wahl J J Virol. 1980; 36(2):622-6.

PMID: 7431490 PMC: 353684. DOI: 10.1128/JVI.36.2.622-626.1980.

A bacteriophage T5 mutant with an increased frequency of single-chain interruptions.

Rhoades M J Virol. 1984; 51(2):553-5.

PMID: 6086964 PMC: 254472. DOI: 10.1128/JVI.51.2.553-555.1984.

References

Sharp P, Sugden B, Sambrook J . Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973; 12(16):3055-63. DOI: 10.1021/bi00740a018. View

Frenkel G, Richardson C . The deoxyribonuclease induced after infection of Escherichia coli by bacteriophage T5. I. Characterization of the enzyme as a 5'-exonuclease. J Biol Chem. 1971; 246(15):4839-47. View

Richardson C . Enzymes in DNA metabolism. Annu Rev Biochem. 1969; 38:795-840. DOI: 10.1146/annurev.bi.38.070169.004051. View

Hayward G . Gel electrophoretic separation of the complementary strands of bacteriophage DNA. Virology. 1972; 49(1):342-4. DOI: 10.1016/s0042-6822(72)80042-4. View

Altman S, Denhardt D . Evidence for preferential breakage of the minus strand of phi-X174 replicative form DNA by a T4-induced endonuclease. Biochim Biophys Acta. 1970; 224(1):21-8. DOI: 10.1016/0005-2787(70)90616-7. View

Thomas Jr C, Rubenstein I . THE ARRANGEMENTS OF NUCLEOTIDE SEQUENCES IN T2 AND T5 BACTERIOPHAGE DNA MOLECULES. Biophys J. 1964; 4:93-106. PMC: 1367458. DOI: 10.1016/s0006-3495(64)86771-0. View

Moyer R, Buchanan J . Effect of calcium ions on synthesis of T5-specific proteins. J Biol Chem. 1970; 245(22):5897-903. View

Henry T, Knippers R . Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease. Proc Natl Acad Sci U S A. 1974; 71(4):1549-53. PMC: 388268. DOI: 10.1073/pnas.71.4.1549. View

Rhoades M . Cleavage of T5 DNA by the Escherichia coli R-I restriction endonuclease. Virology. 1975; 64(1):170-9. DOI: 10.1016/0042-6822(75)90089-6. View

10.

Jacquemin-Sablon A, Richardson C . Analysis of the interruptions in bacteriophage T5 DNA. J Mol Biol. 1970; 47(3):477-93. DOI: 10.1016/0022-2836(70)90316-5. View

11.

Carrington J, LUNT M . Studies on the replication of bacteriophage T5. J Gen Virol. 1973; 18(2):91-109. DOI: 10.1099/0022-1317-18-2-91. View

12.

Rhoades M, Rhoades E . Terminal repetition in the DNA of bacteriophage T5. J Mol Biol. 1972; 69(2):187-200. DOI: 10.1016/0022-2836(72)90224-0. View

13.

Lanni Y . First-step-transfer deoxyribonucleic acid of bacteriophage T5. Bacteriol Rev. 1968; 32(3):227-42. PMC: 408296. DOI: 10.1128/br.32.3.227-242.1968. View

14.

Frenkel G, Richardson C . The deoxyribonuclease induced after infection of Escherichia coli by bacteriophage T5. II. Role of the enzyme in replication of the pahge deoxyribonucleic acid. J Biol Chem. 1971; 246(15):4848-52. View

15.

Durwald H, HOFFMANN-BERLING H . Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants. J Mol Biol. 1968; 34(2):331-46. DOI: 10.1016/0022-2836(68)90257-x. View

16.

Hayward G, Smith M . The chromosome of bacteriophage T5. II. Arrangement of the single-stranded DNA fragments in the T5 + and T5st(O) chromosomes. J Mol Biol. 1972; 63(3):397-407. DOI: 10.1016/0022-2836(72)90436-6. View

17.

Hayward G, Smith M . The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972; 63(3):383-95. DOI: 10.1016/0022-2836(72)90435-4. View

18.

Altman S, Meselson M . A T4-induced endonuclease which attacks T4 DNA. Proc Natl Acad Sci U S A. 1970; 66(3):716-21. PMC: 283109. DOI: 10.1073/pnas.66.3.716. View

19.

Bujard H . Location of single-strand interruptions in the DNA of bacteriophage T5. Proc Natl Acad Sci U S A. 1969; 62(4):1167-74. PMC: 223629. DOI: 10.1073/pnas.62.4.1167. View

20.

Lang D . Molecular weights of coliphages and coliphage DNA. 3. Contour length and molecular weight of DNA from bacteriophages T4, T5 and T7, and from bovine papilloma virus. J Mol Biol. 1970; 54(3):557-65. DOI: 10.1016/0022-2836(70)90126-9. View