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Tumour-necrosis Factor from the Rabbit. IV. Purification and Chemical Characterization

Overview
Journal Br J Cancer
Specialty Oncology
Date 1980 Sep 1
PMID 7426345
Citations 11
Authors
N Matthews
H C Ryley
M L Neale
Affiliations
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Abstract

Serum from rabbits with BCG/endotoxin-induced shock is growth inhibitory or cytotoxic to a range ot tumour cell lines. The active component, tumour-necrosis factor (TNF), has been purified 1000-fold by sequential salt precipitation, ion-exchange chromatography and gel-filtration. TNF had a mol. wt of 67,000 on gradient PAGE and 39,000 on Ultrogel AcA44 gel-filtration. The isoelectric point was pH 5.1-5.2. TNF was susceptible to the proteolytic enzyme pronase, but resistant to trypsin or papain. On isopycnic ultracentrifugation it had a buoyant density of 1.27, confirming that it is protein in nature, with little or no carbohydrate. This is also suggested by its failure to bind to a range of lectins.

Citing Articles

The Physiological Role of Tumor Necrosis Factor in Human Immunity and Its Potential Implications in Spinal Manipulative Therapy: A Narrative Literature Review.

Zhang L, Yao C J Chiropr Med. 2016; 15(3):190-6.

PMID: 27660595 PMC: 5021902. DOI: 10.1016/j.jcm.2016.04.016.

Tumour-necrosis factor from the rabbit. V. Synthesis in vitro by mononuclear phagocytes from various tissues of normal and BCG-injected rabbits.

Matthews N Br J Cancer. 1981; 44(3):418-24.

PMID: 7284238 PMC: 2010759. DOI: 10.1038/bjc.1981.200.

Production of an anti-tumour cytotoxin by human monocytes.

Matthews N Immunology. 1981; 44(1):135-42.

PMID: 7275181 PMC: 1555127.

Physicochemical characterization of cytostatic factors released from human monocytes.

Nissen-Meyer J, Hammerstrom J Infect Immun. 1982; 38(1):67-73.

PMID: 7141697 PMC: 347698. DOI: 10.1128/iai.38.1.67-73.1982.

Anti-tumour cytotoxin from macrophages: no correlation between cytotoxin adsorption by tumour cell lines and their cytotoxin susceptibility.

Matthews N Immunology. 1984; 53(3):537-43.

PMID: 6490095 PMC: 1454925.

References
1.
Agrawal B, Goldstein I . Protein-carbohydrate interaction. VI. Isolation of concanavalin A by specific adsorption on cross-linked dextran gels. Biochim Biophys Acta. 1967; 147(2):262-71. View
2.
Margolis J, Kenrick K . Polyacrylamide gel electrophoresis in a continuous molecular sieve gradient. Anal Biochem. 1968; 25(1):347-62. DOI: 10.1016/0003-2697(68)90109-7. View
3.
Nicolson G, BLAUSTEIN J . The interaction of Ricinus communis agglutinin with normal and tumor cell surfaces. Biochim Biophys Acta. 1972; 266(2):543-7. DOI: 10.1016/0005-2736(72)90109-5. View
4.
CARSWELL E, Old L, Kassel R, Green S, Fiore N, Williamson B . An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975; 72(9):3666-70. PMC: 433057. DOI: 10.1073/pnas.72.9.3666. View
5.
Vretblad P . Purification of lectins by biospecific affinity chromatography. Biochim Biophys Acta. 1976; 434(1):169-76. DOI: 10.1016/0005-2795(76)90047-7. View