Degradation of Intracellular DNA in KB Cells Infected with Cyt Mutants of Human Adenovirus Type 12

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1981 Oct 1

PMID 7288923

Citations 23

Authors

H Ezoe

R B Fatt

S Mak

Affiliations

Soon will be listed here.

Abstract

A group of mutants (cyt mutants) with much reduced oncogenicity was isolated from the highly oncogenic human adenovirus type 12 (Takemori et al., Virology 36: 575-586, 1968). These mutants induce extensive cellular destruction during lytic infection of human cells and produce low yields of virions. We report here that human KB cells infected with cyt mutants synthesized a reduced amount of viral DNA as compared with cells infected with the parental virus. Furthermore, the newly synthesized viral and cellular DNAs were extensively degraded in mutant-infected cells. Viral DNA was first synthesized as complete genome size, and most of it was degraded to subgenomic size within 6 h after synthesis. This virus-induced DNA degradation function, as well as the low yield of virions, was prevented by co-infection with the parental virus.

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References

Warmaar S, Cohen J . A quantitative assay for DNA-DNA hybrids using membrane filters. Biochem Biophys Res Commun. 1966; 24(4):554-8. DOI: 10.1016/0006-291x(66)90356-1. View

Takemori N, Riggs J, Aldrich C . Genetic studies with tumorigenic adenoviruses. I. Isolation of cytocidal (cyt) mutants of adenovirus type 12. Virology. 1968; 36(4):575-86. DOI: 10.1016/0042-6822(68)90189-x. View

Takemori N, Riggs J, Aldrich C . Genetic studies with tumorigenic adenoviruses. II. Heterogeneity of cyt mutants of adenovirus type 12. Virology. 1969; 38(1):8-15. DOI: 10.1016/0042-6822(69)90122-6. View

Mak S . Transcription and replication of viral deoxyribonucleic acid in cells coinfected with adenovirus types 2 and 12. J Virol. 1969; 4(5):651-6. PMC: 375921. DOI: 10.1128/JVI.4.5.651-656.1969. View

Burlingham B, DOERFLER W, Pettersson U, Philipson L . Adenovirus endonuclease: association with the penton of adenovirus type 2. J Mol Biol. 1971; 60(1):45-64. DOI: 10.1016/0022-2836(71)90446-3. View

Takemori N . Genetic studies with tumorigenic adenoviruses. 3. Recombination in adenovirus type 12. Virology. 1972; 47(1):157-67. DOI: 10.1016/0042-6822(72)90249-8. View

Burlingham B, DOERFLER W . An endonuclease in cells infected with adenovirus and associated with adenovirions. Virology. 1972; 48(1):1-13. DOI: 10.1016/0042-6822(72)90108-0. View

Mak S, Mak I . Viral DNA synthesis in cells infected with ultraviolet-irradiated human adenovirus type 2. Biochim Biophys Acta. 1974; 340(2):117-29. DOI: 10.1016/0005-2787(74)90104-x. View

Ezoe H, Mak S . Comparative studies on functions of human adenovirus type 12 and its low oncogenic mutant virions. J Virol. 1974; 14(4):733-9. PMC: 355576. DOI: 10.1128/JVI.14.4.733-739.1974. View

10.

Marusyk R, Morgan A, Wadell G . Association of endonuclease activity with serotypes belonging to the three subgroups of human adenoviruses. J Virol. 1975; 16(2):456-8. PMC: 354685. DOI: 10.1128/JVI.16.2.456-458.1975. View

11.

Ortin J, Scheidtmann K, Greenberg R, Westphal M, DOERFLER W . Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells. J Virol. 1976; 20(2):355-72. PMC: 355004. DOI: 10.1128/JVI.20.2.355-372.1976. View

12.

Nass K, Frenkel G . Adenovirus-induced inhibition of cellular DNase. J Virol. 1978; 26(2):540-3. PMC: 354092. DOI: 10.1128/JVI.26.2.540-543.1978. View

13.

SMILEY J, Mak S . Transcription map for adenovirus type 12 DNA. J Virol. 1978; 28(1):227-39. PMC: 354262. DOI: 10.1128/JVI.28.1.227-239.1978. View

14.

Jones N, Shenk T . Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. Cell. 1979; 17(3):683-9. DOI: 10.1016/0092-8674(79)90275-7. View

15.

Padmanabhan R, Stabel S, Winterhoff U, DOERFLER W . Specificity and mode of cleavage of the pH 4.0 endonuclease from adenovirus type 2 - infected KB cells. Nucleic Acids Res. 1979; 6(9):3161-76. PMC: 327923. DOI: 10.1093/nar/6.9.3161. View

16.

DHalluin J, Allart C, Cousin C, Boulanger P, Martin G . Adenovirus early function required for protection of viral and cellular DNA. J Virol. 1979; 32(1):61-71. PMC: 353527. DOI: 10.1128/JVI.32.1.61-71.1979. View

17.

Tsang L, Marusyk R . Purification and partial characterization of a 33 000 molecular weight endonuclease associated with human adenovirus type 5. Can J Microbiol. 1980; 26(10):1224-31. DOI: 10.1139/m80-204. View

18.

Green M, Pina M . Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus. Virology. 1963; 20:199-207. DOI: 10.1016/0042-6822(63)90157-0. View

19.

Pereira M, MACCALLUM F . INFECTION WITH ADENOVIRUS TYPE 12. Lancet. 1964; 1(7326):198-9. DOI: 10.1016/s0140-6736(64)92290-1. View