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Transcription Map for Adenovirus Type 12 DNA

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Journal J Virol
Date 1978 Oct 1
PMID 702649
Citations 7
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Abstract

The regions of the adenovirus type 12 genome which encode l- and r-strand-specific cytoplasmic RNA were mapped by the following procedure. Radioactive, intact, separated complementary strands of the viral genome were hybridized to saturating amounts of unlabeled late cytoplasmic RNA. The segments of each DNA strand complementary to the RNA were then purified by S1 nuclease digestion of the hybrids. The arrangement of the coding regions of each strand was deduced from the pattern of hybridization of these probes to unlabeled viral DNA fragments produced by digestion with EcoRI, BamHI, and HindIII.. The resulting map is similar, if not identical, to that of adenovirus type 2. The subset of the late cytoplasmic RNA sequences which are expressed at early times were located on the map by hybridizing labeled, early cytoplasmic RNA to both unlabeled DNA fragments and unlabeled complementary strands of specific fragments. Early cytoplasmic RNA hybridized to the r-strand to EcoRI-C and BamHI-B and to the l-strand of BamHI-E. Hybridization to BamHI-C was also observed. The relative rates of accumulation of cytoplasmic RNA complementary to individual restriction fragments was measured at both early and late times. Early during infection, most of the viral RNA appearing in the cytoplasm was derived from the molecular ends of the genome. Later (24 to 26 h postinfection) the majority of the newly labeled cytoplasmic RNA was transcribed from DNA sequences mapping between 25 and 60 map units on the genome.

Citing Articles

Mapping of adenovirus 12 mRNA's transcribed from the transforming region.

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PMID: 7463554 PMC: 353691. DOI: 10.1128/JVI.36.3.639-651.1980.


Degradation of intracellular DNA in KB cells infected with cyt mutants of human adenovirus type 12.

Ezoe H, Fatt R, Mak S J Virol. 1981; 40(1):20-7.

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Complementation of adenovirus type 5 host range mutants by adenovirus type 12 in coinfected HeLa and BHK-21 cells.

Rowe D, Graham F J Virol. 1981; 38(1):191-7.

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Mapping of an adenovirus function involved in the inhibition of DNA degradation.

Lai Fatt R, Mak S J Virol. 1982; 42(3):969-77.

PMID: 7097865 PMC: 256930. DOI: 10.1128/JVI.42.3.969-977.1982.


Transformation of rat cells by cyt mutants of adenovirus type 12 and mutants of adenovirus type 5.

Mak I, Mak S J Virol. 1983; 45(3):1107-17.

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