Specific Receptor Binding of Staphylococcal Enterotoxins by Murine Splenic Lymphocytes

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1981 Sep 1

PMID 7287185

Citations 5

Authors

S Buxser

P F Bonventre

D L Archer

Affiliations

Soon will be listed here.

Abstract

We describe a reliable assay to measure the specific binding of 125I-labeled staphylococcal enterotoxin A (SEA) by murine spleen cells. Toxin binding by lymphocytes was specific in that it was inhibited by unlabeled SEA but not by unrelated proteins. The biological activity of SEA (T-lymphocyte mitogenesis) correlated with toxin binding to splenic lymphocytes. In the presence of high concentrations of [125I]SEA, specific binding increased rapidly and approached saturation after 2 h. Toxin binding was sensitive to temperature and pH and was directly proportional to the concentration of spleen cells in the incubation mixture. We estimated that there was a single class of toxin-binding sites, which had an apparent equilibrium dissociation constant (Kd) of 8 x 10(-7) M and numbered 3,600 sites per cell. SEA and the antigenically distinct compounds staphylococcal enterotoxins B and E in excess competitively inhibited binding of [125I]SEA to mouse spleen cells. Our data suggest a common class of binding sites for the three staphylococcal enterotoxins.

Citing Articles

Binding and activation of major histocompatibility complex class II-deficient macrophages by staphylococcal exotoxins.

Beharka A, Armstrong J, Iandolo J, Chapes S Infect Immun. 1994; 62(9):3907-15.

PMID: 8063407 PMC: 303047. DOI: 10.1128/iai.62.9.3907-3915.1994.

Effects of staphylococcal enterotoxin A on the rat gastrointestinal tract.

Beery J, Taylor S, Schlunz L, Freed R, Bergdoll M Infect Immun. 1984; 44(2):234-40.

PMID: 6370862 PMC: 263506. DOI: 10.1128/iai.44.2.234-240.1984.

Cell surface molecules involved in early events in T-cell mitogenic stimulation by staphylococcal enterotoxins.

Vroegop S, Buxser S Infect Immun. 1989; 57(6):1816-24.

PMID: 2785962 PMC: 313361. DOI: 10.1128/iai.57.6.1816-1824.1989.

Selective proliferation of natural killer cells among monocyte-depleted peripheral blood mononuclear cells as a result of stimulation with staphylococcal enterotoxin B.

Garcia-Penarrubia P, Lennon M, Koster F, Kelley R, Bankhurst A Infect Immun. 1989; 57(7):2057-65.

PMID: 2731983 PMC: 313841. DOI: 10.1128/iai.57.7.2057-2065.1989.

Superantigens: biology, immunology, and potential role in disease.

Drake C, Kotzin B J Clin Immunol. 1992; 12(3):149-62.

PMID: 1400895 DOI: 10.1007/BF00918083.

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