Release of Histamine from Hamster Mast Cells by Concanavalin A and Phytohemagglutinin

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1974 May 1

PMID 4132912

Citations 11

Authors

W A Hook

S F Dougherty

J J Oppenheim

Affiliations

Soon will be listed here.

Abstract

Concanavalin A (Con A) and phytohemagglutinin (PHA) released histamine from hamster mast cells incubated in a serum-free medium. Concentrations of Con A and PHA approximating those optimal for transforming lymphocytes also released maximal amounts of histamine without apparent cytotoxicity. Higher concentrations of mitogen inhibited both lymphocyte transformation and histamine release. Incubation at 37 C for 15 min released histamine, although longer times were more effective. Supernatants from cultured hamster splenocytes stimulated with Con A also released histamine from added mast cells. However, the effect could be inhibited by the addition of 0.1 M methyl alpha-D-mannoside or by passing the spleen cell culture supernatants through Sephadex G-75 to remove Con A. This mitogen-induced release of mast cell histamine is therefore not mediated by a lymphokine but probably results from a direct interaction of mitogens with receptors on mast cells.

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References

Singh S, Tevethia S . In vitro stimulation of hamster lymphocytes with concanavalin A. Infect Immun. 1972; 5(3):339-45. PMC: 422372. DOI: 10.1128/iai.5.3.339-345.1972. View

UVNAS B, THON I . Isolation of "biologically intact" mast cells. Exp Cell Res. 1959; 18:512-20. DOI: 10.1016/0014-4827(59)90316-7. View

Williams J, Bell R . Membrane matrix disruption by melittin. Biochim Biophys Acta. 1972; 288(2):255-62. DOI: 10.1016/0005-2736(72)90246-5. View

Hook W, Snyderman R, Mergenhagen S . Histamine-releasing factor generated by the interaction of endotoxin with hamster serum. Infect Immun. 1970; 2(4):462-7. PMC: 416033. DOI: 10.1128/iai.2.4.462-467.1970. View

Powell A, Leon M . Reversible interaction of human lymphocytes with the mitogen concanavalin A. Exp Cell Res. 1970; 62(2):315-25. DOI: 10.1016/0014-4827(70)90560-4. View

RANADIVE N, Cochrane C . Mechanism of histamine release from mast cells by cationic protein (band 2) from neutrophil lysosomes. J Immunol. 1971; 106(2):506-16. View

Nowell P . Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes. Cancer Res. 1960; 20:462-6. View

Bach M, BLOCH K, Austen K . IgE and IgGa antibody-mediated release of histamine from rat peritoneal cells. I. Optimum conditions for in vitro preparation of target cells with antibody and and challenge with antigen. J Exp Med. 1971; 133(4):752-71. PMC: 2138970. DOI: 10.1084/jem.133.4.752. View

OSLER A, RANDALL H, Hill B, OVARY Z . Studies on the mechanism of hypersensitivity phenomena. III. The participation of complement in the formation of anaphylatoxin. J Exp Med. 1959; 110(2):311-39. PMC: 2136993. DOI: 10.1084/jem.110.2.311. View

10.

Hook W, Snyderman R, Mergenhagen S . Further characterization of a factor from endotoxin-treated serum which releases histamine and heparin from mast cells. Infect Immun. 1972; 5(6):909-14. PMC: 422462. DOI: 10.1128/iai.5.6.909-914.1972. View

11.

Ishizaka T, Ishizaka K, JOHANSSON S, Bennich H . Histamine release from human leukocytes by anti-gamma E antibodies. J Immunol. 1969; 102(4):884-92. View

12.

Lichtenstein L . The immediate allergic response: in vitro separation of antigen activation, decay and histamine release. J Immunol. 1971; 107(4):1122-30. View

13.

Keller R . Interrelations between different types of cells. II. Histamine-release from the mast cells of various species by cationic polypeptides of polymorphonuclear leukocyte lysosomes and other cationic compounds. Int Arch Allergy Appl Immunol. 1968; 34(2):139-44. View

14.

Keller R . Concanavalin A, a model "antigen" for the in vitro detection of cell-bound reaginic antibody in the rat. Clin Exp Immunol. 1973; 13(1):139-47. PMC: 1553749. View

15.

Canty T, Wunderlich J . Quantitative in vitro assay of cytotoxic cellular immunity. J Natl Cancer Inst. 1970; 45(4):761-72. View

16.

Oppenheim J, Wolstencroft R, Gell P . Delayed hypersensitivity in the guinea-pig to a protein-hapten conjugate and its relationship to in vitro transformation of lymph node, spleen, thymus and peripheral blood lymphocytes. Immunology. 1967; 12(1):89-102. PMC: 1409006. View

17.

Lichtenstein L, OSLER A . STUDIES ON THE MECHANISMS OF HYPERSENSITIVITY PHENOMENA. IX. HISTAMINE RELEASE FROM HUMAN LEUKOCYTES BY RAGWEED POLLEN ANTIGEN. J Exp Med. 1964; 120:507-30. PMC: 2137776. DOI: 10.1084/jem.120.4.507. View