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Detection of Chromosome Variation in Interphase by in Situ Hybridization with Repetitive DNA Probes: Potential Applications to Cytogenetic Analysis and Mutagenicity Testing

Overview
Journal Cytotechnology
Specialties Biotechnology
Genetics
Date 2012 Feb 24
PMID 22358434
Authors
E Raimondi
S Scariolo
P Vagnarelli
A De Sario
L De Carli
Affiliations
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Abstract

Individual chromosomes can be identified by means of in situ hybridization with DNA probes for chromosome-specific repetitive sequences. The efficiency and sensitivity of the method are strictly dependent on the characteristics of the probes and the experimental conditions. Using three probes with different copy numbers, we demonstrated that the target chromosomes can be visualized in interphase when the homologous sequences are repeated at least 50 times.Possible applications of interphase analysis to clinical cytogenetics and mutagenicity testing are discussed.

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