The Influence of Hyaluronidase on Urinary and Renal Medullary Composition Following Antidiuretic Stimulus in the Rat

1. The influence of urinary hyaluronidase (believed to be predominantly of renal origin) on the urinary concentrating process has been studied in rats subjected to antidiuretic stimulus. 2. Antiserum against a partially purified preparation of this enzyme has been raised in rabbits. Urinary volume, solute excretion and medullary composition have been investigated in rats treated with this antiserum (0.2 ml./100 g body weight, i.v.) before water deprivation for 48 hr or infusion for up to 4 hr with arginine-vasopressin. Control rats were pre-treated with normal rabbit serum. 3. Pre-treatment with antiserum against rat urinary hyaluronidase (AUase) caused water-deprived rats to excrete urine at a rate significantly greater, and of osmolality significantly lower, than that recorded in control rats. 4. The increase in medullary solute gradient which typically accompanies antidiuresis was significantly reduced in water-deprived rats pre-treated with AUase. 5. In rats treated with AUase and infused for 4 hr with arginine-vasopressin, there was no significant increase in the medullary solute gradient, whereas this increased markedly in control rats. 6. During the first 24 hr of water deprivation there weas an increase in the rate of Ca excretion by control rats which was abolished by pre-treatment with AUase. 7. The effects of antiserum against a partially purified preparation of rat testicular hyaluronidase (ATase) were studied in water-deprived rats. No evidence was obtained that this enzyme has any influence on renal function. 8. It is concluded that urinary hyaluronidase, but not testicular hyaluronidase, plays an important role in facilitating the urinary concentrating process following antidiuretic stimulus.