Studies on Bone Enzymes. The Assay of Acid Hydrolases and Other Enzymes in Bone Tissue

Overview

Journal Biochem J

Specialty Biochemistry

Date 1965 Nov 1

PMID 16749142

Citations 44

Authors

G Vaes

P Jacques

Affiliations

Soon will be listed here.

Abstract

1. Nine acid hydrolases, cytochrome oxidase, alkaline phenylphosphatase and catalase were demonstrated in 0.25m-sucrose homogenates of newborn-rat calvaria. The acid hydrolases were: acid phenylphosphatase, acid beta-glycerophosphatase, beta-glucuronidase, beta-N-acetylglucosaminidase (beta-N-acetylaminodeoxyglucosidase), acid ribonuclease and acid deoxyribonuclease, showing optimum activity at about pH5; cathepsin, beta-galactosidase and hyaluronidase, with optimum activity at about pH3.6. 2. The main kinetic characters of these enzymes have been studied and methods for their quantitative assay have been worked out. The activities present in bone are given and compared with those found in liver. 3. Acid-phosphatase activity was assayed with phenyl phosphate and beta-glycerophosphate as substrates: activities with these two substrates appeared to be due to two different enzymes. Acid phenylphosphatase is particularly labile and is readily inactivated by various physical or chemical agents.

Citing Articles

Enhancing pH Modulation and Calcium Ions Release in External Resorption Artificial Defects: A Comparative Analysis of Ultrasonic Activation Effects on Ca(OH), MTA, and CEM Cement.

Kheradyar A, Adel M, Sirati-Sabet M, Kolahdouzan A, Shafagh S Int J Dent. 2024; 2024:8850548.

PMID: 39574827 PMC: 11581801. DOI: 10.1155/2024/8850548.

Studies on bone enzymes. The activation and release of latent acid hydrolases and catalase in bone-tissue homogenates.

Vaes G Biochem J. 1965; 97(2):393-402.

PMID: 16749144 PMC: 1264654. DOI: 10.1042/bj0970393.

Studies on bone enzymes. Distribution of acid hydrolases, alkaline phenylphosphatase, cytochrome oxidase and catalase in subcellular fraction of bone tissue homogenates.

Vaes G, Jacques P Biochem J. 1965; 97(2):389-92.

PMID: 16749143 PMC: 1264653. DOI: 10.1042/bj0970389.

Effect of clodronate on established adjuvant arthritis.

Osterman T, Kippo K, Lauren L, Hannuniemi R, SELLMAN R Rheumatol Int. 1994; 14(4):139-47.

PMID: 7871332 DOI: 10.1007/BF00579699.

Effect of clodronate on established collagen-induced arthritis in rats.

Osterman T, Kippo K, Lauren L, Hannuniemi R, SELLMAN R Inflamm Res. 1995; 44(6):258-63.

PMID: 7583522 DOI: 10.1007/BF01782979.

References

Tolksdorf S . The in vitro determination of hyaluronidase. Methods Biochem Anal. 1954; 1:425-57. DOI: 10.1002/9780470110171.ch15. View

DE DUVE C, PRESSMAN B, GIANETTO R, WATTIAUX R, APPELMANS F . Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955; 60(4):604-17. PMC: 1216159. DOI: 10.1042/bj0600604. View

REISSIG J, STORMINGER J, LELOIR L . A modified colorimetric method for the estimation of N-acetylamino sugars. J Biol Chem. 1955; 217(2):959-66. View

Kind P, King E . Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol. 1954; 7(4):322-6. PMC: 1023845. DOI: 10.1136/jcp.7.4.322. View

Nordin B . [The solubility of powdered bone]. J Biol Chem. 1957; 227(2):551-64. View

Tonna E . Histologic and histochemical studies on the periosteum of male and female rats at different ages. J Gerontol. 1958; 13(1):14-9. DOI: 10.1093/geronj/13.1.14. View

Borle A, Nichols N, Nichols Jr G . Metabolic studies of bone in vitro. II. The metabolic patterns of accretion and resorption. J Biol Chem. 1960; 235:1211-4. View

Holt S, Hicks R . Studies on formalin fixation for electron microscopy and cytochemical staining purposes. J Biophys Biochem Cytol. 1961; 11:31-45. PMC: 2225110. DOI: 10.1083/jcb.11.1.31. View

VAES G, Nichols Jr G . Effects of a dose of parathyroid extract on bone metabolic pathways. Endocrinology. 1962; 70:546-55. DOI: 10.1210/endo-70-4-546. View

10.

Cameron D . The fine structure of bone and calcified cartilage. A critical review of the contribution of electron microscopy to the understading of osteogenesis. Clin Orthop Relat Res. 1963; 26:199-228. View

11.

APPELMANS F, DE DUVE C . Tissue fractionation studies. 3. Further observations on the binding of acid phosphatase by rat-liver particles. Biochem J. 1955; 59(3):426-33. PMC: 1216261. DOI: 10.1042/bj0590426. View

12.

GIANETTO R, DE DUVE C . Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles. Biochem J. 1955; 59(3):433-8. PMC: 1216262. DOI: 10.1042/bj0590433. View

13.

APPELMANS F, WATTIAUX R, DE DUVE C . Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver. Biochem J. 1955; 59(3):438-45. PMC: 1216263. DOI: 10.1042/bj0590438. View

14.

SCHLAGER F . [Occurrence and localization of beta-D-galactosidase in bone, cartilage and adjacent tissue of white mice]. Acta Histochem. 1959; 8:176-84. View

15.

Sellinger O, BEAUFAY H, Jacques P, Doyen A, DE DUVE C . Tissue fractionation studies. 15. Intracellular distribution and properties of beta-N-acetylglucosaminidase and beta-galactosidase in rat liver. Biochem J. 1960; 74:450-6. PMC: 1204241. DOI: 10.1042/bj0740450. View

16.

SCHARTUM S, Nichols Jr G . Concerning pH gradients between the extracellular compartment and fluids bathing the bone mineral surface and their relation to calcium ion distribution. J Clin Invest. 1962; 41:1163-8. PMC: 291024. DOI: 10.1172/JCI104569. View

17.

COOPERSTEIN S, LAZAROW A . A microspectrophotometric method for the determination of cytochrome oxidase. J Biol Chem. 1951; 189(2):665-70. View

18.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

19.

Park J, Johnson M . A submicrodetermination of glucose. J Biol Chem. 1949; 181(1):149-51. View

20.

Vaes G, Jacques P . Studies on bone enzymes. Distribution of acid hydrolases, alkaline phenylphosphatase, cytochrome oxidase and catalase in subcellular fraction of bone tissue homogenates. Biochem J. 1965; 97(2):389-92. PMC: 1264653. DOI: 10.1042/bj0970389. View