Differential Giemsa Staining of Sister Chromatids After Extraction with Acids

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1977 Nov 14

PMID 72637

Citations 15

Authors

S Takayama

S Sakanishi

Affiliations

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Abstract

Chromosomes of Chinese hamster strain cells were air-dried on slides after BrdU substitution for two or three rounds of replication. The preparations were treated with 20% PCA at 55 degrees C for 20-30 min, or 5N HCl at 55 degrees C for 15-20 min. After staining with Giemsa, unifilarly BrdU-substituted chromatids stained faintly and bifilarly substituted chromatids stained darkly. Such a pattern of sister chromatid differential staining was confirmed by the examination of metaphase cells grown with BrdU for three rounds of replication.

Citing Articles

Human cells in suspension 3. The effect of in vitro aging on metaphase chromosome contraction in human lymphoid cells.

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Topographic examination of sister chromatid differential staining by Nomarski interference microscopy and scanning electron microscopy.

Takayama S, UTSUMI K, Sasaki Y Chromosoma. 1981; 82(1):113-9.

PMID: 7261710 DOI: 10.1007/BF00285754.

Sister chromatid exchange analysis.

LATT S, Schreck R Am J Hum Genet. 1980; 32(3):297-313.

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On the mechanism of differential Giemsa staining of BrdU-substituted chromatids.

Gonzalez-Gil G, Navarrete M Chromosoma. 1982; 86(3):375-82.

PMID: 6184205 DOI: 10.1007/BF00292264.

DNA extraction during Giemsa differentiation of chromatids singly and doubly substituted with BrdU.

Webber L, Brasch J, Smyth D Chromosoma. 1981; 81(5):691-700.

PMID: 6162624 DOI: 10.1007/BF00329580.

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