Down-regulation of the Sodium Pump Following Chronic Exposure of HeLa Cells and Chick Embryo Heart Cells to Ouabain

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1981 Jun 1

PMID 7236988

Citations 6

Authors

J F Aiton

J F Lamb

P Ogden

Affiliations

Soon will be listed here.

Abstract

1 HeLa cells and primary cultures of embryonic chick heart cells were grown in medium containing low concentrations of ouabain for 24 h. 2 Compared with normal cells, cells grown in ouabain have fewer free sodium pump sites, an increased intracellular sodium concentration and a decreased intracellular potassium concentration. The cells are able to maintain their intracellular ion contents because the remaining pump sites have an increased turnover rate. 3 When cells that have been chronically exposed to ouabain are returned to normal growth medium, the sodium pump site numbers increase; the recovery process begins within 6 to 8 h and is complete within 24 h. Recovery of pump site numbers is primarily dependent upon de novo protein synthesis since the protein synthesis inhibitor, cycloheximide, prevents recovery.

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