A Photoinduced Cleavage of DNA Useful for Determining T Residues

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1982 Dec 20

PMID 7162986

Citations 3

Authors

A Simoncsits

I Torok

Affiliations

Soon will be listed here.

Abstract

Irradiation of 5'-[32P]-phosphate labeled DNA fragments with ultraviolet light in the presence of primary amines followed by piperidine treatment resulted in base-specific cleavage of the DNA chain at T residues, accompanied by a less intensive G reaction. This simple, T greater than G cleavage offers an alternative method for determining T residues in chemical DNA sequencing.

Citing Articles

A new procedure for determining thymine residues in DNA sequencing. Photoinduced cleavage of DNA fragments in the presence of spermine.

Saito I, Sugiyama H, Matsuura T, Ueda K, Komano T Nucleic Acids Res. 1984; 12(6):2879-85.

PMID: 6324131 PMC: 318712. DOI: 10.1093/nar/12.6.2879.

Structure and organization of the chicken H2B histone gene family.

Grandy D, Dodgson J Nucleic Acids Res. 1987; 15(3):1063-80.

PMID: 3822819 PMC: 340508. DOI: 10.1093/nar/15.3.1063.

The chicken carbonic anhydrase II gene: evidence for a recent shift in intron position.

Yoshihara C, Lee J, Dodgson J Nucleic Acids Res. 1987; 15(2):753-70.

PMID: 3029691 PMC: 340465. DOI: 10.1093/nar/15.2.753.

References

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