Morphology of the Intermediate Stages in the Lamellar to Hexagonal Lipid Phase Transition

Overview

Journal J Membr Biol

Date 1982 Jan 1

PMID 7143432

Citations 4

Authors

V L Borovjagin

J A Vergara

T J McIntosh

Affiliations

Soon will be listed here.

Abstract

The addition of calcium to suspensions of egg phosphatidylcholine and cardiolipin converts multiwalled liposomes to the hexagonal (HII) phase (Rand, R.P., Sengupta, S. (1972) Biochim. Biophys. Acta 255:484-492). We have studied this lamellar to hexagonal phase transition by freeze-fracture, thin-section electron microscopy, and X-ray diffraction and have morphologically characterized the intermediate stages. The first step in the transition involves the invagination and fusion of bilayers, marked by the appearance of lipidic intramembrane particles and "crater-like" indentations, as the large liposomes are converted to smaller flattened and elongated vesicles. The next step is the formation of tightly packed hexagonal arrays of tubules, each tubule being about 11 to 15 nm in diameter. These tubules are filled with fluid and a lipid bilayer forms the wall of each cylinder. Finally this tubular bilayer phase is converted to the hexagonal (HII) phase, where the distance between tubes is 5.5 to 7.5 nm.

Citing Articles

Lamellar-to-hexagonalII phase transitions in the plasma membrane of isolated protoplasts after freeze-induced dehydration.

Gordon-Kamm W, Steponkus P Proc Natl Acad Sci U S A. 1984; 81(20):6373-7.

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Lamellar to tubular conformational changes in the endoplasmic reticulum of the retinal pigment epithelium of the newt, Notophthalmus viridescens.

Yorke M, Dickson D Cell Tissue Res. 1985; 241(3):629-37.

PMID: 4028144 DOI: 10.1007/BF00214585.

Inverted micellar intermediates and the transitions between lamellar, cubic, and inverted hexagonal lipid phases. I. Mechanism of the L alpha----HII phase transitions.

Siegel D Biophys J. 1986; 49(6):1155-70.

PMID: 3719074 PMC: 1329699. DOI: 10.1016/S0006-3495(86)83744-4.

Calcium-induced fusion of didodecylphosphate vesicles: the lamellar to hexagonal II (HII) phase transition.

Rupert L, van Breemen J, van Bruggen E, Engberts J, Hoekstra D J Membr Biol. 1987; 95(3):255-63.

PMID: 3585980 DOI: 10.1007/BF01869487.

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