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A Rapid Quantitative Assay for Activated Neutrophils

Overview
Journal Inflammation
Specialties Allergy & Immunology
Pathology
Date 1982 Sep 1
PMID 7129599
Citations 8
Authors
W W Bullock
H R CREAMER
W L GABLER
Affiliations
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Abstract

We present here a rapid, sensitive, and convenient assay for activated human PMNs based on detecting the decreased optical density (OD) of aggregated cell suspensions. This quantitative assay uses an ELIZA machine to measure OD changes, with time, of activated cells (5 X 10(5) cells/well) in microtiter plates. The assay is sensitive, detecting aggregation induced by as little as 0.0001 microgram/ml of LPS, or lymphokines in Con-A-activated supernatant diluted 1/500. The assay permits analysis of 400 separate PMN suspensions on the same day starting with less than 80 ml of blood.

Citing Articles

Phorbol myristate acetate induction of chemotactic migration of human polymorphonuclear neutrophils.

GABLER W, Bullock W, CREAMER H Inflammation. 1993; 17(4):521-30.

PMID: 8406693 DOI: 10.1007/BF00916591.

Endogenous component chemotactic assay (ECCA).

CREAMER H, GABLER W, Bullock W Inflammation. 1983; 7(4):321-9.

PMID: 6654473 DOI: 10.1007/BF00916296.

Activated rat neutrophils. A sequential quantitative assay for aggregation and NBT reduction.

Fennell K, CREAMER H, GABLER W, Brown A, Bullock W Inflammation. 1984; 8(3):287-300.

PMID: 6333396 DOI: 10.1007/BF00916417.

Role of extracellular calcium in neutrophil responsiveness to chemotactic tripeptides.

GABLER W, CREAMER H, Bullock W Inflammation. 1986; 10(3):281-92.

PMID: 3091496 DOI: 10.1007/BF00916123.

Aggregated human colostral sIgA stimulates delayed, non-complement-dependent, NBT reduction by human neutrophils.

Bullock W, Wang Y, GABLER W, CREAMER H Inflammation. 1989; 13(1):67-78.

PMID: 2921084 DOI: 10.1007/BF00918964.

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