Energy Linked Uptake of Demethylphalloin by Isolated Rat Liver Cells

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1982 Apr 1

PMID 7110372

Citations 2

Authors

E Petzinger

M Frimmer

Affiliations

Soon will be listed here.

Abstract

Isolated hepatocytes accumulate demethylphalloin (DMP) under aerobic conditions. In the absence of oxygen the initial rate of the DMP uptake is reduced to less than 20%, while reoxygenation restores the transport. Liver cells release previously accumulated phallotoxin when the oxygen supply is interrupted. DMP uptake is blocked by oligomycin, antimycin A, carbonylcyano-chlorophenylhydrazon (CCCP) or dinitrophenol and is partially inhibited by carboxyatractyloside. Depletion of ATP in hepatocytes by replacement of glucose by fructose reduces the accumulation of toxin too. Below 22 degrees C no uptake was measurable. Between 22 degrees and 37 degrees C an apparent activation energy of 76.6 kJ/mol toxin and a Q10 of 2.6 was calculated for the carrier mediated uptake of DMP. The results suggest that the uptake of demethylphalloin is an energy dependent substrate transfer very similar to that of cholate.

Citing Articles

Lack of intestinal transport of [3H]-demethylphalloin: comparative studies with phallotoxins and bile acids on isolated small intestinal cells and ileal brush border membrane vesicles.

Petzinger E, Burckhardt G, Schwenk M, Faulstich H Naunyn Schmiedebergs Arch Pharmacol. 1982; 320(2):196-200.

PMID: 7121618 DOI: 10.1007/BF00506321.

Common properties of hepatocellular uptake of cholate, iodipamide and antamanide, as distinct from the uptake of bromosulfophthalein.

Petzinger E, Joppen C, Frimmer M Naunyn Schmiedebergs Arch Pharmacol. 1983; 322(2):174-9.

PMID: 6688124 DOI: 10.1007/BF00512393.

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