Mechanism of Regulation of Glucose Transport in Rhizobium Leguminosarum

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Mar 1

PMID 7061388

Citations 5

Authors

G E de Vries

A A Van Brussel

A QUISPEL

Affiliations

Soon will be listed here.

Abstract

Multiple glucose transport systems were distinguished in Rhizobium leguminosarum. We found nonlinear Lineweaver-Burk plots for the uptake of glucose, 2-deoxy-D-glucose, and alpha-methyl-D-glucoside, and this implied the existence of at least two uptake mechanisms. Different patterns of inhibition of 2-deoxy-D-glucose uptake and alpha-methyl-D-glucoside uptake at 0.1 mM by various carbohydrates revealed differences in the stereospecificities of the transport systems. Osmotic shock treatment abolished transport activities, and two independent glucose-binding activities were detected in the supernatants. Induction of glucose transport was repressed strongly by L-malate, even in the presence of excess D-glucose. Rhizobium bacteroids showed no significant glucose uptake activity at different oxygen concentrations. These results suggested that glucose transport is repressed by dicarboxylic acids during R. leguminosarum symbiosis.

Citing Articles

Sequential uptake of aldoses over fructose and enhanced phosphate solubilization in Rhizobium sp. RM.

Champaneria A, Iyer B, Rajkumar S Appl Microbiol Biotechnol. 2022; 106(11):4251-4268.

PMID: 35661910 DOI: 10.1007/s00253-022-11997-w.

Succinate transport by free-living forms of Rhizobium japonicum.

McAllister C, Lepo J J Bacteriol. 1983; 153(3):1155-62.

PMID: 6402487 PMC: 221758. DOI: 10.1128/jb.153.3.1155-1162.1983.

Evaluation of active versus passive uptake of metabolites by Rhizobium japonicum bacteroids.

Reibach P, Streeter J J Bacteriol. 1984; 159(1):47-52.

PMID: 6203891 PMC: 215590. DOI: 10.1128/jb.159.1.47-52.1984.

Kinetics of nutrient-limited transport and microbial growth.

Button D Microbiol Rev. 1985; 49(3):270-97.

PMID: 3930934 PMC: 373036. DOI: 10.1128/mr.49.3.270-297.1985.

Effects of 2,4-dichlorophenoxyacetic acid on Rhizobium sp. membrane fluidity.

Fabra de Peretti A, Duffard R, Evangelista de Duffard A Arch Environ Contam Toxicol. 1992; 23(3):301-6.

PMID: 1456775 DOI: 10.1007/BF00216237.

References

KUNDIG W, Roseman S . Sugar transport. I. Isolation of a phosphotransferase system from Escherichia coli. J Biol Chem. 1971; 246(5):1393-406. View

Furlong C, Morris R, Kandrach M, Rosen B . A multichamber equilibrium dialysis apparatus. Anal Biochem. 1972; 47(2):514-26. DOI: 10.1016/0003-2697(72)90146-7. View

Neal J . Analysis of Michaelis kinetics for two independent, saturable membrane transport functions. J Theor Biol. 1972; 35(1):113-8. DOI: 10.1016/0022-5193(72)90196-8. View

Hylemon P, Phibbs Jr P . Independent regulation of hexose catabolizing enzymes and glucose transport activity in Pseudomonas aeruginosa. Biochem Biophys Res Commun. 1972; 48(5):1041-8. DOI: 10.1016/0006-291x(72)90813-3. View

Miyairi S, Fukui S . Glucose-1-phosphate-negative mutant of Agrobacterium tumefaciens. J Bacteriol. 1973; 113(2):658-65. PMC: 285278. DOI: 10.1128/jb.113.2.658-665.1973. View

Ng F, DAWES E . Chemostat studies on the regulation of glucose metabolism in Pseudomonas aeruginosa by citrate. Biochem J. 1973; 132(2):129-40. PMC: 1177573. DOI: 10.1042/bj1320129. View

Midgley M, DAWES E . The regulation of transport of glucose and methyl alpha-glucoside in Pseudomonas aeruginosa. Biochem J. 1973; 132(2):141-54. PMC: 1177574. DOI: 10.1042/bj1320141. View

Mukkada A, Long G, Romano A . The uptake of 2-deoxy-D-glucose by Pseudomonas aeruginosa and its regulation. Biochem J. 1973; 132(2):155-62. PMC: 1177575. DOI: 10.1042/bj1320155. View

Lo T, Rayman M, SANWAL B . Transport of succinate in Escherichia coli. III. Biochemical and genetic studies of the mechanism of transport in membrane vesicles. Can J Biochem. 1974; 52(10):854-66. DOI: 10.1139/o74-122. View

10.

Lynch W, MacLeod J, Franklin M . Effect of temperature on the activity and synthesis of glucose-catabolizing enzymes in Pseudomonas fluorescens. Can J Microbiol. 1975; 21(10):1560-72. DOI: 10.1139/m75-229. View

11.

Whiting P, Midgley M, DAWES E . The role of glucose limitation in the regulation of the transport of glucose, gluconate and 2-oxogluconate, and of glucose metabolism in Pseudomonas aeruginosa. J Gen Microbiol. 1976; 92(2):304-10. DOI: 10.1099/00221287-92-2-304. View

12.

Stinson M, Cohen M, MERRICK J . Purification and properties of the periplasmic glucose-binding protein of Pseudomonas aeruginosa. J Bacteriol. 1977; 131(2):672-81. PMC: 235478. DOI: 10.1128/jb.131.2.672-681.1977. View

13.

Laane C, Haaker H, Veeger C . Involvement of the cytoplasmic membrane in nitrogen fixation by Rhizobium leguminosarum bacteroids. Eur J Biochem. 1978; 87(1):147-53. DOI: 10.1111/j.1432-1033.1978.tb12361.x. View

14.

Ucker D, Signer E . Catabolite-repression-like phenomenon in Rhizobium meliloti. J Bacteriol. 1978; 136(3):1197-200. PMC: 218559. DOI: 10.1128/jb.136.3.1197-1200.1978. View

15.

Arias A, Cervenansky C, Gardiol A, Martinez-Drets G . Phosphoglucose isomerase mutant of Rhizobium meliloti. J Bacteriol. 1979; 137(1):409-14. PMC: 366610. DOI: 10.1128/jb.137.1.409-414.1979. View

16.

Lim S, Shanmugam K . Regulation of hydrogen utilisation in Rhizobium japonicum by cyclic AMP. Biochim Biophys Acta. 1979; 584(3):479-92. DOI: 10.1016/0304-4165(79)90121-1. View

17.

Bewick M, Lo T . Dicarboxylic acid transport in Escherichia coli K12: involvement of a binding protein in the translocation of dicarboxylic acids across the outer membrane of the cell envelope. Can J Biochem. 1979; 57(6):653-61. DOI: 10.1139/o79-082. View

18.

Ronson C, Primrose S . Effect of glucose on polyol metabolism by Rhizobium trifolii. J Bacteriol. 1979; 139(3):1075-8. PMC: 218061. DOI: 10.1128/jb.139.3.1075-1078.1979. View

19.

Stumpf D, BURRIS R . A micromethod for the purification and quantification of organic acids of the tricarboxylic acid cycle in plant tissues. Anal Biochem. 1979; 95(1):311-5. DOI: 10.1016/0003-2697(79)90221-5. View

20.

Courtois B, Hornez J, DERIEUX J . [Effect of 2-ketogluconic acid synthesis on the exopolysaccharide production in a Rhizobium meliloti strain]. Can J Microbiol. 1979; 25(10):1191-6. View