Metabolism of [3H]-(+/-)-isoprenaline by Isolated Atria and Coronary Arteries of the Kitten

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1980 Mar 1

PMID 7052341

Citations 5

Authors

E J CORNISH

R G Goldie

Affiliations

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Abstract

1 Isolated coronary arteries of the kitten accumulated more unchanged isoprenaline and metabolized more amine than atria following incubation for 1 to 20 min with [3H]-(+/-)-isoprenaline (25 ng/ml or 5 micrograms/ml). 2 Cortisol (10 or 80 microM), U-0521 (120 microM) and oxytetracycline (100 microM) all reduced metabolite formation. 3 Cortisol inhibited 'Iso InfluxMin' (cellular isoprenaline accumulation plus total metabolite production). In contrast, it increased, decreased or did not alter accumulation of unmetabolized isoprenaline, depending upon the experimental conditions. 4 Isoprenaline accumulation was increased in atria and reduced in coronary arteries by U-0521, while oxytetracycline reduced accumulation in coronary arteries at the high amine concentration. 5 It is concluded that in atria, cortisol inhibits metabolism and has differential effects on a number of extraneuronal compartments which accumulate isoprenaline. Both cortisol and U-0521 appear to be extraneuronal uptake inhibitors and inhibitors of catechol-O-methyltransferase in coronary arteries. Oxytetracycline may have effects additional to inhibition of isoprenaline binding to connective tissue fibres.

Citing Articles

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Accumulation of 3H-(-)noradrenaline in the rabbit aorta not related to uptake1 and uptake2, but sensitive to 3,4-dihydroxy-2-methylpropiophenone (U-0521) and oxytetracycline.

Henseling M Naunyn Schmiedebergs Arch Pharmacol. 1983; 323(2):121-7.

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The extraneuronal compartments for the distribution of isoprenaline in the rat heart.

Trendelenburg U, Fleig H, Bryan L, Bonisch H Naunyn Schmiedebergs Arch Pharmacol. 1983; 324(3):169-79.

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Effect of steroids on beta-adrenoceptor-mediated relaxation of pig bronchus.

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Effect of hypothermia on beta 1-adrenoceptor-mediated relaxation of pig bronchus.

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