Demonstration of High Molecular Weight Poly (adenosine Diphosphate Ribose)

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1978 Sep 1

PMID 704350

Citations 5

Authors

M Tanaka

K Hayashi

H Sakura

M Miwa

T Matsushima

T Sugimura

Affiliations

Soon will be listed here.

Abstract

An electrophoretic system was established that resolves poly(adenosine diphosphate ribose), enzymatically synthesized polymer from NAD+, by size difference of one residue on polyacrylamide gel. The existence of a polymer of at least 65 residues was demonstrated by band counting in this system. The polymer showed a heterogeneous size distribution on the electrophoregram, and the molecular weight of the largest polymer was deduced to be more than 4.5 X 10(5) daltons. The discrepancy between the size, estimated by electrophoresis, and the chain length, determined by the ratio of total radioactivity to that derived from the terminus, suggests that the polymer has a branched structure.

Citing Articles

Activation of the abundant nuclear factor poly(ADP-ribose) polymerase-1 by Helicobacter pylori.

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Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions.

DAmours D, Desnoyers S, DSilva I, Poirier G Biochem J. 1999; 342 ( Pt 2):249-68.

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Poly(ADP-ribose): historical perspective.

Sugimura T, Miwa M Mol Cell Biochem. 1994; 138(1-2):5-12.

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ADP-ribosyltransferase in Plasmodium (malaria parasites).

Okolie E, Onyezili N Biochem J. 1983; 209(3):687-93.

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Structure of poly(adenosine diphosphate ribose): identification of 2'-[1''-ribosyl-2''-(or 3''-)(1'''-ribosyl)]adenosine-5',5'',5'''-tris(phosphate) as a branch linkage.

Miwa M, Saikawa N, Yamaizumi Z, Nishimura S, Sugimura T Proc Natl Acad Sci U S A. 1979; 76(2):595-9.

PMID: 218210 PMC: 382995. DOI: 10.1073/pnas.76.2.595.

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