Structure of Poly(adenosine Diphosphate Ribose): Identification of 2'-[1''-ribosyl-2''-(or 3''-)(1'''-ribosyl)]adenosine-5',5'',5'''-tris(phosphate) As a Branch Linkage

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Feb 1

PMID 218210

Citations 52

Authors

M Miwa

N Saikawa

Z Yamaizumi

S Nishimura

T Sugimura

Affiliations

Soon will be listed here.

Abstract

Poly([14C]adenosine diphosphate ribose) was synthesized from [14C]NAD+ with calf thymus nuclei. The fraction containing poly(adenosine diphosphate ribose) eluted with 0.22--0.40 M phosphate buffer (pH 6.8) from a hydroxylapatite column, was completely hydrolyzed with venom phosphodiesterase, and was separated by DEAE-Sephadex A-25 column chromatography in 7 M urea. A new compound, which constituted 2% of the products from poly(adenosine diphosphate ribose), was found in addition to the expected products--i.e., 5'-AMP, 2'-(1''-ribosyl)adenosine-5',5''-bis(phosphate), and its derivatives. This compound was identified as 2'-[1''-ribosyl 2''-(or 3''-)(1'''-ribosyl)]adenosine-5',5'',5'''-tris(phosphate). The existence of this compound is evidence of a branching structure of poly(adenosine diphosphate ribose), which was previously thought to be a linear molecule. The content of this compound suggests that the frequency of branching is about 1 per 20--30 adenosine diphosphate ribose residues of high molecular weight poly(adenosine diphosphate ribose).

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References

Ames B, DUBIN D . The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960; 235:769-75. View

Park J, Johnson M . A submicrodetermination of glucose. J Biol Chem. 1949; 181(1):149-51. View

Rushizky G, BARTOS E, SOBER H . CHROMATOGRAPHY OF MIXED OLIGONUCLEOTIDES ON DEAE-SEPHADEX. Biochemistry. 1964; 3:626-9. DOI: 10.1021/bi00893a005. View

Rushizky G, SOBER H . Desalting of mono- and oligonucleotides. Biochim Biophys Acta. 1962; 55:217. DOI: 10.1016/0006-3002(62)90950-2. View

DISCHE Z, Borenfreund E . A new color reaction for the determination of aldopentose in presence of other saccharides. Biochim Biophys Acta. 1957; 23(3):639-42. DOI: 10.1016/0006-3002(57)90387-6. View

Bock R, LING N, Morell S, Lipton S . Ultraviolet absorption spectra of adenosine-5'-triphosphate and related 5'-ribonucleotides. Arch Biochem Biophys. 1956; 62(2):253-64. DOI: 10.1016/0003-9861(56)90123-0. View

Ferro A, Oppenheimer N . Structure of a poly (adenosine diphosphoribose) monomer: 2'-(5"-hosphoribosyl)-5'-adenosine monophosphate. Proc Natl Acad Sci U S A. 1978; 75(2):809-13. PMC: 411346. DOI: 10.1073/pnas.75.2.809. View

Miwa M, Saito H, Sakura H, Saikawa N, Watanabe F, Matsushima T . A 13C NMR study of poly(adenosine diphosphate ribose) and its monomers: evidence of alpha-(1'' leads to 2') ribofuranosy1 ribofuranoside risidue. Nucleic Acids Res. 1977; 4(11):3997-4005. PMC: 343216. DOI: 10.1093/nar/4.11.3997. View

Smulson M, Schein P, Mullins Jr D, Sudhakar S . A putative role for nicotinamide adenine dinucleotide-promoted nuclear protein modification in the antitumor activity of N-methyl-N-nitrosourea. Cancer Res. 1977; 37(9):3006-12. View

10.

HAYAISHI O, Ueda K . Poly(ADP-ribose) and ADP-ribosylation of proteins. Annu Rev Biochem. 1977; 46:95-116. DOI: 10.1146/annurev.bi.46.070177.000523. View

11.

Hilz H, Stone P . Poly(ADP-ribose) and ADP-ribosylation of proteins. Rev Physiol Biochem Pharmacol. 1976; 76:1-58, 177. DOI: 10.1007/BFb0027686. View

12.

Oka J, Ueda K, HAYAISHI O . Snake venom phosphodiesterase: simple purification with Blue Sepharose and its application to poly(ADP-ribose) study. Biochem Biophys Res Commun. 1978; 80(4):841-8. DOI: 10.1016/0006-291x(78)91321-9. View

13.

Tanaka M, Miwa M, Hayashi K, Kubota K, Matsushima T . Separation of oligo(adenosine diphosphate ribose) fractions with various chain lengths and terminal structures. Biochemistry. 1977; 16(7):1485-89. DOI: 10.1021/bi00626a037. View

14.

Miwa M, Oda K, Segawa K, Tanaka M, Irie S . Cell density-dependent increase in chromatin-associated ADP-ribosyltransferase activity in simian virus 40-transformed cells. Arch Biochem Biophys. 1977; 181(1):313-21. DOI: 10.1016/0003-9861(77)90510-0. View

15.

Sakura H, Miwa M, Tanaka M, Kanai Y, Shimada T, Matsushima T . Natural occurence of a biopolymer, poly (adenosine diphosphate ribose). Nucleic Acids Res. 1977; 4(8):2903-15. PMC: 342618. DOI: 10.1093/nar/4.8.2903. View

16.

Tanaka M, Hayashi K, Sakura H, Miwa M, Matsushima T, Sugimura T . Demonstration of high molecular weight poly (adenosine diphosphate ribose). Nucleic Acids Res. 1978; 5(9):3183-94. PMC: 342240. DOI: 10.1093/nar/5.9.3183. View

17.

Stone P, Lorimer 3rd W, Kidwell W . Properties of the complex between histone H1 and poly(ADP-ribose synthesised in HeLa cell nuclei. Eur J Biochem. 1977; 81(1):9-18. DOI: 10.1111/j.1432-1033.1977.tb11921.x. View

18.

Kanai Y, Kawaminami Y, Miwa M, Matsushima T, Sugimura T . Naturally-occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus. Nature. 1977; 265(5590):175-7. DOI: 10.1038/265175a0. View

19.

Sugimura T, Yoshimura N, Miwa M, Nagai H, Nagao M . Studies on poly(adenosine diphosphate-ribose). XI. Purification of poly(adenosine diphosphate-ribose) on a hydroxylapatite column. Arch Biochem Biophys. 1971; 147(2):660-5. DOI: 10.1016/0003-9861(71)90425-5. View

20.

Harada F, Kimura F, Nishimura S . Primary sequence of tRNA val from Escherichia coli B. I. Oligonucleotide sequences of digests of Escherichia coli tRNA val with RNase T and pancreatic RNase. Biochemistry. 1971; 10(17):3269-77. DOI: 10.1021/bi00793a017. View