The Catabolite-sensitive Promoter for the Chloramphenicol Acetyl Transferase Gene is Preceded by Two Binding Sites for the Catabolite Gene Activator Protein

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Apr 1

PMID 7037748

Citations 19

Authors

S F Le Grice

H Matzura

R Marcoli

S Iida

T A Bickle

Affiliations

Soon will be listed here.

Abstract

DNase I protection experiments have indicated that the cyclic AMP-catabolite gene activator protein complex binds to two regions preceding the chloramphenicol acetyl transferase (cat) gene in Escherichia coli. One of these lies adjacent to the RNA polymerase binding site, whereas the second lies approximately 130 base pairs upstream from the starting point of transcription. Additional DNase protection experiments and in vitro transcription experiments with modified templates indicate that the catabolite gene activator protein site proximal to the cat promoter functions independently of the distal site, indicating that in vitro the second of these sites is not required for transcriptional activation of the cat gene.

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References

Pastan I, Adhya S . Cyclic adenosine 5'-monophosphate in Escherichia coli. Bacteriol Rev. 1976; 40(3):527-51. PMC: 413971. DOI: 10.1128/br.40.3.527-551.1976. View

Dickson R, Abelson J, Johnson P . Nucleotide sequence changes produced by mutations in the lac promoter of Escherichia coli. J Mol Biol. 1977; 111(1):65-75. DOI: 10.1016/s0022-2836(77)80132-0. View

Musso R, Di Lauro R, Adhya S, de Crombrugghe B . Dual control for transcription of the galactose operon by cyclic AMP and its receptor protein at two interspersed promoters. Cell. 1977; 12(3):847-54. DOI: 10.1016/0092-8674(77)90283-5. View

Bolivar F, Rodriguez R, Greene P, Betlach M, Heyneker H, Boyer H . Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977; 2(2):95-113. View

Chang A, Cohen S . Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978; 134(3):1141-56. PMC: 222365. DOI: 10.1128/jb.134.3.1141-1156.1978. View

Bolivar F . Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978; 4(2):121-36. DOI: 10.1016/0378-1119(78)90025-2. View

Schmitz A, Galas D . The interaction of RNA polymerase and lac repressor with the lac control region. Nucleic Acids Res. 1979; 6(1):111-37. PMC: 327677. DOI: 10.1093/nar/6.1.111. View

Johnsrud L . DNA sequence of the transposable element IS1. Mol Gen Genet. 1979; 169(2):213-8. DOI: 10.1007/BF00271673. View

Taniguchi T, ONeill M, de Crombrugghe B . Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters. Proc Natl Acad Sci U S A. 1979; 76(10):5090-4. PMC: 413085. DOI: 10.1073/pnas.76.10.5090. View

10.

Alton N, Vapnek D . Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9. Nature. 1979; 282(5741):864-9. DOI: 10.1038/282864a0. View

11.

Mallick U, Herrlich P . Regulation of synthesis of a major outer membrane protein: cyclic AMP represses Escherichia coli protein III synthesis. Proc Natl Acad Sci U S A. 1979; 76(11):5520-3. PMC: 411680. DOI: 10.1073/pnas.76.11.5520. View

12.

Marcoli R, Iida S, Bickle T . The DNA sequence of an IS/-flanked transposon coding for resistance to chloramphenicol and fusidic acid. FEBS Lett. 1980; 110(1):11-4. DOI: 10.1016/0014-5793(80)80011-1. View

13.

Laskey R . The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. Methods Enzymol. 1980; 65(1):363-71. DOI: 10.1016/s0076-6879(80)65047-2. View

14.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

15.

Le Grice S, Matzura H . Localisation of the transcription initiation site of the chloramphenicol resistance gene on plasmid pAC184. FEBS Lett. 1980; 113(1):42-6. DOI: 10.1016/0014-5793(80)80490-x. View

16.

Siebenlist U, Simpson R, Gilbert W . E. coli RNA polymerase interacts homologously with two different promoters. Cell. 1980; 20(2):269-81. DOI: 10.1016/0092-8674(80)90613-3. View

17.

Simpson R . Interaction of the cAMP receptor protein with the lac promoter. Nucleic Acids Res. 1980; 8(4):759-66. PMC: 327308. View

18.

Schmitz A . Cyclic AMP receptor proteins interacts with lactose operator DNA. Nucleic Acids Res. 1981; 9(2):277-92. PMC: 326692. DOI: 10.1093/nar/9.2.277. View

19.

Lee N, Gielow W, Wallace R . Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli. Proc Natl Acad Sci U S A. 1981; 78(2):752-6. PMC: 319880. DOI: 10.1073/pnas.78.2.752. View

20.

ONeill M, Amass K, de Crombrugghe B . Molecuar model of the DNA interaction site for the cyclic AMP receptor protein. Proc Natl Acad Sci U S A. 1981; 78(4):2213-7. PMC: 319314. DOI: 10.1073/pnas.78.4.2213. View