A Transcriptional Terminator Sequence in the Prokaryotic Transposable Element IS1

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1987 Mar 1

PMID 3035345

Citations 9

Authors

P Hubner

S Iida

W Arber

Affiliations

Soon will be listed here.

Abstract

The prokaryotic transposable element IS1 is known to exert a strong polar effect upon integration into an operon. To elucidate this polar effect, we constructed a plasmid which has an IS1 integrated between the 5' half of the tet gene for tetracycline resistance and the cat structural gene for chloramphenicol resistance. The cat gene is expressed by the tet promoter and the presence of IS1 in orientation I, in which the IS1 transposase genes insA and insB are in the same orientation as the cat gene, reduced the cat expression. By introducing deletions or insertions within the IS1 sequence, we were able to map a rho-dependent terminator TIS1A between the insA and insB genes. Translational interruption between these ins genes is important for TIS1A to be an active terminator.

Citing Articles

Novel miniature transposable elements in thermophilic Synechococcus strains and their impact on an environmental population.

Nelson W, Bhaya D, Heidelberg J J Bacteriol. 2012; 194(14):3636-42.

PMID: 22563047 PMC: 3393498. DOI: 10.1128/JB.00333-12.

Analysis of insertion sequences in thermophilic cyanobacteria: exploring the mechanisms of establishing, maintaining, and withstanding high insertion sequence abundance.

Nelson W, Wollerman L, Bhaya D, Heidelberg J Appl Environ Microbiol. 2011; 77(15):5458-66.

PMID: 21666019 PMC: 3147441. DOI: 10.1128/AEM.05090-11.

A new regulatory circuit in ribosomal protein operons: S2-mediated control of the rpsB-tsf expression in vivo.

Aseev L, Levandovskaya A, Tchufistova L, Scaptsova N, Boni I RNA. 2008; 14(9):1882-94.

PMID: 18648071 PMC: 2525966. DOI: 10.1261/rna.1099108.

Insertion sequences.

Mahillon J, Chandler M Microbiol Mol Biol Rev. 1998; 62(3):725-74.

PMID: 9729608 PMC: 98933. DOI: 10.1128/MMBR.62.3.725-774.1998.

Mutational analysis of the open reading frames in the transposable element IS1.

Jakowec M, Prentki P, Chandler M, Galas D Genetics. 1988; 120(1):47-55.

PMID: 2851480 PMC: 1203504. DOI: 10.1093/genetics/120.1.47.

References

de Crombrugghe B, Adhya S, Gottesman M, Pastan I . Effect of Rho on transcription of bacterial operons. Nat New Biol. 1973; 241(113):260-4. DOI: 10.1038/newbio241260a0. View

Morgan W, Bear D, Litchman B, von Hippel P . RNA sequence and secondary structure requirements for rho-dependent transcription termination. Nucleic Acids Res. 1985; 13(10):3739-54. PMC: 341270. DOI: 10.1093/nar/13.10.3739. View

Close T, Rodriguez R . Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements. Gene. 1982; 20(2):305-16. DOI: 10.1016/0378-1119(82)90048-8. View

Messing J, Vieira J . A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982; 19(3):269-76. DOI: 10.1016/0378-1119(82)90016-6. View

Peden K . Revised sequence of the tetracycline-resistance gene of pBR322. Gene. 1983; 22(2-3):277-80. DOI: 10.1016/0378-1119(83)90112-9. View

Tinoco Jr I, Borer P, Dengler B, Levin M, Uhlenbeck O, Crothers D . Improved estimation of secondary structure in ribonucleic acids. Nat New Biol. 1973; 246(150):40-1. DOI: 10.1038/newbio246040a0. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Reeve J . Use of minicells for bacteriophage-directed polypeptide synthesis. Methods Enzymol. 1979; 68:493-503. DOI: 10.1016/0076-6879(79)68038-2. View

Johnsrud L . DNA sequence of the transposable element IS1. Mol Gen Genet. 1979; 169(2):213-8. DOI: 10.1007/BF00271673. View

10.

Sommer H, Schumacher B, Saedler H . A new type of IS1-mediated deletion. Mol Gen Genet. 1981; 184(2):300-7. DOI: 10.1007/BF00272921. View

11.

Le Grice S, Matzura H, Marcoli R, Iida S, Bickle T . The catabolite-sensitive promoter for the chloramphenicol acetyl transferase gene is preceded by two binding sites for the catabolite gene activator protein. J Bacteriol. 1982; 150(1):312-8. PMC: 220115. DOI: 10.1128/jb.150.1.312-318.1982. View

12.

Starlinger P . IS elements and transposons. Plasmid. 1980; 3(3):241-59. DOI: 10.1016/0147-619x(80)90039-6. View

13.

Starlinger P, Saedler H . IS-elements in microorganisms. Curr Top Microbiol Immunol. 1976; 75:111-52. DOI: 10.1007/978-3-642-66530-1_4. View

14.

Christen A, Pall M, Manzara T, Lurquin P . Rapid isolation of Escherichia coli minicells by glass-fiber filtration: study of plasmid-coded polypeptides. Gene. 1983; 23(2):195-8. DOI: 10.1016/0378-1119(83)90051-3. View

15.

Machida Y, Machida C, Ohtsubo E . Insertion element IS1 encodes two structural genes required for its transposition. J Mol Biol. 1984; 177(2):229-45. DOI: 10.1016/0022-2836(84)90454-6. View

16.

Ohtsubo H, Ohtsubo E . Nucleotide sequence of an insertion element, IS1. Proc Natl Acad Sci U S A. 1978; 75(2):615-9. PMC: 411306. DOI: 10.1073/pnas.75.2.615. View

17.

Besemer J, Herpers M . Suppression of polarity of insertion mutations within the gal operon of E. coli. Mol Gen Genet. 1977; 151(3):295-304. DOI: 10.1007/BF00268793. View

18.

Iida S, Arber W . Transposable element IS1 intrinsically generates target duplications of variable length. Proc Natl Acad Sci U S A. 1985; 82(3):839-43. PMC: 397142. DOI: 10.1073/pnas.82.3.839. View