Determination of Base Pairing in Yeast 5S and 5.8S RNA Infrared Spectroscopy

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1981 Aug 11

PMID 7024920

Citations 4

Authors

J Stulz

T Ackermann

B Appel

V A Erdmann

Affiliations

Soon will be listed here.

Abstract

Infrared Spectroscopy was used to determine the numbers of base pairs for yeast 5S RNA and 5.8S RNA. The spectra were recorded at 20 degrees C and 50 degrees C, where tertiary interactions are assumed to be of less importance. It may be concluded that the structure of both RNAs is highly ordered and that there are large contributions of tertiary interactions. The results are compared with data derived from structural models that were proposed in the literature as well as with data previously published for prokaryotic 5S RNAs.

Citing Articles

Melting of local ordered structures in yeast 5S ribosomal RNA in aqueous salts.

Ohta S, Maruyama S, Nitta K, Sugai S Nucleic Acids Res. 1983; 11(10):3363-73.

PMID: 6344010 PMC: 325969. DOI: 10.1093/nar/11.10.3363.

The 5S RNA genes of Schizosaccharomyces pombe.

Mao J, Appel B, Schaack J, Sharp S, Yamada H, Soll D Nucleic Acids Res. 1982; 10(2):487-500.

PMID: 6278416 PMC: 326152. DOI: 10.1093/nar/10.2.487.

RNA structure analysis using T2 ribonuclease: detection of pH and metal ion induced conformational changes in yeast tRNAPhe.

Vary C, Vournakis J Nucleic Acids Res. 1984; 12(17):6763-78.

PMID: 6207483 PMC: 320115. DOI: 10.1093/nar/12.17.6763.

Three-dimensional structural model of eubacterial 5S RNA that has functional implications.

Pieler T, Erdmann V Proc Natl Acad Sci U S A. 1982; 79(15):4599-603.

PMID: 6181508 PMC: 346722. DOI: 10.1073/pnas.79.15.4599.

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