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Microtest Procedure for Isolation of Chlamydia Trachomatis

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1981 Jun 1
PMID 7019235
Citations 37
Authors
B L Yoder
W E Stamm
C M Koester
E R ALEXANDER
Affiliations
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Abstract

Management of patients potentially infected with Chlamydia trachomatis has been hampered by the cost and time required to perform Chlamydia cultures. To isolate C. trachomatis, we developed a microtiter method that exhibited equal sensitivity but less frequent contamination than our previously used vial-cover slip culture system. In addition, costs and technician time were substantially reduced with the microtest method. Subsequent studies showed that cycloheximide-treated cells were superior to 5-iodo-2-deoxyuridine-treated cells in the microtest method and that a subpassage significantly enhanced the sensitivity of the method. The microtest method appears to be a sensitive, rapid, and economical method for isolating C. trachomatis.

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