Determination of Human Immunoglobulin M Rheumatoid Factor by a Solid-phase Radioimmunoassay Which Uses Human Immunoglobulin G in Antigen-antibody Complexes

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1978 Aug 1

PMID 701459

Citations 9

Authors

B Ziola

O Meurman

M T Matikainen

A Salmi

J L KALLIOMAKI

Affiliations

Soon will be listed here.

Abstract

A solid-phase radioimmunoassay for the rapid determination of human immunoglobulin M (IgM) rheumatoid factor (RF) has been developed. Preparation of the solid phase for the assay involved the formation of complexes between respiratory syncytial virus-specific human IgG antibodies and virus antigen on the surface of polystyrene balls. Binding of serum RF to IgG in the immune complex was subsequently detected by 125I-labeled mu-chain-specific antibodies to human IgM. The amount of radioactive indicator antibody bound was converted to units of RF by comparison to the standard curve for an RF reference-serum pool. This assay should prove useful in studies of the physiological role of RF, since it can effectively measure low levels of circulating RF.

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