Mapping of Two Transcription Mutations (tlnI and TlnII) Conferring Thiolutin Resistance, Adjacent to DnaZ and Rho in Escherichia Coli

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Jan 1

PMID 7007825

Citations 10

Authors

N Sivasubramanian

R Jayaraman

Affiliations

Soon will be listed here.

Abstract

Two mutations in Escherichia coli conferring resistance to the transcription initiation inhibitor, thiolutin, have been mapped. One of these mutations (tln-I)( maps at 10.2 min on the genetic map and is cotransducible with dnaZ at a frequency of approximately 50%. The other mutation (tln-II) maps between metE and ilvD, probably close to rho, and is cotransducible with ilvD at a frequency of approximately 65%. The presence of both the mutations in the same cell confers resistance to thiolutin in minimal medium. Either one of them alone renders the cell 'conditionally auxotrophic' in the presence of the drug. The implications of these findings are discussed in relation to the mode of action of the thiolutin sensitive factors in transcription.

Citing Articles

Dithiolopyrrolones: biosynthesis, synthesis, and activity of a unique class of disulfide-containing antibiotics.

Li B, Wever W, Walsh C, Bowers A Nat Prod Rep. 2014; 31(7):905-23.

PMID: 24835149 PMC: 4132845. DOI: 10.1039/c3np70106a.

Use of mutator strains for characterization of novel antimicrobial agents.

ONeill A, Chopra I Antimicrob Agents Chemother. 2001; 45(5):1599-600.

PMID: 11372639 PMC: 90517. DOI: 10.1128/AAC.45.1.1599-1600.2001.

Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Berlyn M Microbiol Mol Biol Rev. 1998; 62(3):814-984.

PMID: 9729611 PMC: 98936. DOI: 10.1128/MMBR.62.3.814-984.1998.

Functions of the gene products of Escherichia coli.

Riley M Microbiol Rev. 1993; 57(4):862-952.

PMID: 7508076 PMC: 372942. DOI: 10.1128/mr.57.4.862-952.1993.

Participation of the host protein(s) in the morphogenesis of bacteriophage P22.

Joshi A, Siddiqui J, Verma M, Chakravorty M Mol Gen Genet. 1982; 186(1):44-9.

PMID: 7050625 DOI: 10.1007/BF00422910.

References

Sirotkin K, Wei J, Snyder L . T4 Bacteriophage-coded RNA polymerase subunit blocks host transcription and unfolds the host chromosome. Nature. 1977; 265(5589):28-32. DOI: 10.1038/265028a0. View

Travers A, Buckland R, Goman M, Le Grice S, Scaife J . A mutation affecting the sigma subunit of RNA polymerase changes transcriptional specificity. Nature. 1978; 273(5661):354-8. DOI: 10.1038/273354a0. View

Khachatourians G, Tipper D . Inhibition of messenger ribonucleic acid synthesis in Escherichia coli by thiolutin. J Bacteriol. 1974; 119(3):795-804. PMC: 245683. DOI: 10.1128/jb.119.3.795-804.1974. View

Tipper D . Inhibition of yeast ribonucleic acid polymerases by thiolutin. J Bacteriol. 1973; 116(1):245-56. PMC: 246415. DOI: 10.1128/jb.116.1.245-256.1973. View

Doi R . Role of ribonucleic acid polymerase in gene selection in procaryotes. Bacteriol Rev. 1977; 41(3):568-94. PMC: 414017. DOI: 10.1128/br.41.3.568-594.1977. View

Chakrabarti S, Maitra P . Development of auxotrophy by streptomycin-resistant mutation. J Bacteriol. 1974; 118(3):1179-80. PMC: 246869. DOI: 10.1128/jb.118.3.1179-1180.1974. View

Ratner D . The interaction bacterial and phage proteins with immobilized Escherichia coli RNA polymerase. J Mol Biol. 1974; 88(2):373-83. DOI: 10.1016/0022-2836(74)90488-4. View

Sivasubramanian N, Jayaraman R . Thiolutin resistant mutants of Escherichia coli are they RNA chain initiation mutants?. Mol Gen Genet. 1976; 145(1):89-96. DOI: 10.1007/BF00331562. View

Low B . Low recombination frequency for markers very near the origin in conjugation in E. coli. Genet Res. 1965; 6(3):469-73. DOI: 10.1017/s0016672300004341. View

10.

Walker J, Henson J, Lee C . Isolation and characterization of plaque-forming lambdadnaZ+ transducing bacteriophages. J Bacteriol. 1977; 130(1):354-65. PMC: 235213. DOI: 10.1128/jb.130.1.354-365.1977. View

11.

Travers A . RNA polymerase specificity and the control of growth. Nature. 1976; 263(5579):641-6. DOI: 10.1038/263641a0. View

12.

Khachatourians G, Tipper D . In vivo effect of thiolutin on cell growth and macromolecular synthesis in Escherichia coli. Antimicrob Agents Chemother. 1974; 6(3):304-10. PMC: 444641. DOI: 10.1128/AAC.6.3.304. View

13.

Bachmann B, Low K, Taylor A . Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976; 40(1):116-67. PMC: 413944. DOI: 10.1128/br.40.1.116-167.1976. View

14.

Das A, Court D, Adhya S . Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho. Proc Natl Acad Sci U S A. 1976; 73(6):1959-63. PMC: 430427. DOI: 10.1073/pnas.73.6.1959. View

15.

Inoko H, Imai M . Isolation and genetic characterization of the nitA mutants of Escherichia coli affecting the termination factor rho. Mol Gen Genet. 1976; 143(2):211-21. DOI: 10.1007/BF00266924. View

16.

Lindahl L, Jaskunas S, Dennis P, Nomura M . Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits. Proc Natl Acad Sci U S A. 1975; 72(7):2743-7. PMC: 432847. DOI: 10.1073/pnas.72.7.2743. View

17.

Morse D, Primakoff P . Relief of polarity in E. coli by "suA". Nature. 1970; 226(5240):28-31. DOI: 10.1038/226028a0. View