New Translocation Sequence Mediating Tetracycline Resistance Found in Escherichia Coli Pathogenic for Piglets

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1980 Jul 1

PMID 6998376

Citations 1

Authors

S T Jorgensen

B Oliva

J Grinsted

P M Bennett

Affiliations

Soon will be listed here.

Abstract

A discrete piece of deoxyribonucleic acid coding for tetracycline (Tc) resistance was found to move from one R plasmid to another in an Escherichia coli strain which is pathogenic for piglets. Since this phenomenon took place also in rec strains, the Tc segment was classified as a transposon and called Tn804. Restriction enzyme analysis with EcoRI, BglII, and HindIII indicated that Tn804 is related to Tn10, a well-known transposon that codes for resistance to tetracycline. Hybridization between plasmids carrying the two transposons provided proof of homology between Tn10 and prt of Tn804. Electron microscopic studies showed a transposon-like structure composed of one loop-stem structure with inverted repetitions of approximately 0.9 megadaltons inserted into the loop of a second loop-stem structure. It is suggested that Tn804 is composed of Tn10 plus another transposable sequence.

Citing Articles

Identification of additional genes on transposon Tn10: tetC and tetD.

Braus G, Argast M, Beck C J Bacteriol. 1984; 160(2):504-9.

PMID: 6094472 PMC: 214762. DOI: 10.1128/jb.160.2.504-509.1984.

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