Autogenous Control of Escherichia Coli Ribosomal Protein L10 Synthesis in Vitro

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1980 May 1

PMID 6994102

Citations 32

Authors

N Brot

P Caldwell

H Weissbach

Affiliations

Soon will be listed here.

Abstract

The DNA-dependent in vitro synthesis of Escherichia coli ribosomal protein L10 was inhibited when L10 was added to the protein-synthesizing incubations. Addition of L10 had little or no effect on the synthesis of ribosomal protein L12, elongation factor Tu (tufB), or the beta and beta' subunits of RNA polymerase. In addition, ribosomal protein L12 did not inhibit its own synthesis or the synthesis of L10. Experiments using a mRNA-directed system showed that the inhibition of the synthesis of L10 by itself is at the level of translation of protein synthesis. The mechanism of inhibition does not appear to be due to increased degradation of L10 mRNA.

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References

Brot N, Marcel R, Yamasaki E, Weissbach H . Further studies on the role of 50 S ribosomal proteins in protein synthesis. J Biol Chem. 1973; 248(20):6952-6. View

Kung H, Spears C, Weissbach H . Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase. J Biol Chem. 1975; 250(4):1556-62. View

Subramanian A . Copies of proteins L7 and L12 and heterogeneity of the large subunit of Escherichia coli ribosome. J Mol Biol. 1975; 95(1):1-8. DOI: 10.1016/0022-2836(75)90330-7. View

Hardy S . The stoichiometry of the ribosomal proteins of Escherichia coli. Mol Gen Genet. 1975; 140(3):253-74. DOI: 10.1007/BF00334270. View

Meyer B, Kleid D, Ptashne M . Lambda repressor turns off transcription of its own gene. Proc Natl Acad Sci U S A. 1975; 72(12):4785-89. PMC: 388816. DOI: 10.1073/pnas.72.12.4785. View

Nomura M, Morgan E . Genetics of bacterial ribosomes. Annu Rev Genet. 1977; 11:297-347. DOI: 10.1146/annurev.ge.11.120177.001501. View

Chu F, Caldwell P, Weissbach H, Brot N . mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu. Proc Natl Acad Sci U S A. 1977; 74(12):5387-91. PMC: 431730. DOI: 10.1073/pnas.74.12.5387. View

Fukuda R, Taketo M, Ishihama A . Autogenous regulation of RNA polymerase beta subunit synthesis in vitro. J Biol Chem. 1978; 253(13):4501-4. View

Lemaire G, Gold L, Yarus M . Autogenous translational repression of bacteriophage T4 gene 32 expression in vitro. J Mol Biol. 1978; 126(1):73-90. DOI: 10.1016/0022-2836(78)90280-2. View

10.

Goldberg G, Caldwell P, Weissbach H, Brot N . In vitro regulation of DNA-dependent synthesis of Escherichia coli ribosomal protein L12. Proc Natl Acad Sci U S A. 1979; 76(4):1716-20. PMC: 383461. DOI: 10.1073/pnas.76.4.1716. View

11.

Dennis P, Fill N . Transcriptional and post-transcriptional control of RNA polymerase and ribosomal protein genes cloned on composite ColE1 plasmids in the bacterium Escherichia coli. J Biol Chem. 1979; 254(16):7540-7. View

12.

Fallon A, Jinks C, Strycharz G, Nomura M . Regulation of ribosomal protein synthesis in Escherichia coli by selective mRNA inactivation. Proc Natl Acad Sci U S A. 1979; 76(7):3411-5. PMC: 383835. DOI: 10.1073/pnas.76.7.3411. View

13.

Jerez C, Goldberg G, Kung H, Huang K, Brot N, Weissbach H . DNA-directed in vitro synthesis of proteins involved in bacterial transcription and translation. Proc Natl Acad Sci U S A. 1979; 76(12):6115-9. PMC: 411813. DOI: 10.1073/pnas.76.12.6115. View

14.

Lindahl L, Zengel J . Operon-specific regulation of ribosomal protein synthesis in Escherichia coli. Proc Natl Acad Sci U S A. 1979; 76(12):6542-6. PMC: 411902. DOI: 10.1073/pnas.76.12.6542. View

15.

Goldberg G, Caldwell P, Weissbach H, Brot N . Regulation of the in vitro synthesis of E. coli ribosomal protein L12. Biochem Biophys Res Commun. 1979; 91(4):1453-61. DOI: 10.1016/0006-291x(79)91229-4. View