Electron Transport to Nitrogenase in Klebsiella Pneumoniae

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1980 May 1

PMID 6994100

Citations 45

Authors

G P Roberts

S Klevickis

W J Brill

Affiliations

Soon will be listed here.

Abstract

Cell-free extracts of nifF and nifJ mutants of Klebsiella pneumoniae are unable to couple acetylene reduction (N2 fixation) by nitrogenase to the oxidation of organic metabolites. However, nifF and nifJ mutants can complement each other in vitro to establish the coupling. This indicates that the products of the nifF and nifJ genes constitute essential elements of the physiological electron pathway to nitrogenase. The electron-transfer-active product of the nifF gene, a flavoprotein, has been purified.

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References

Yoch D . Electron transport carriers involved in nitrogen fixation by the coliform, Klebsiella pneumoniae. J Gen Microbiol. 1974; 83(0):153-64. DOI: 10.1099/00221287-83-1-153. View

Benemann J, Yoch D, Valentine R, Arnon D . The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier. Proc Natl Acad Sci U S A. 1969; 64(3):1079-86. PMC: 223346. DOI: 10.1073/pnas.64.3.1079. View

Nagatani H, Brill W . Nitrogenase V. The effect of Mo, W and V on the synthesis of nitrogenase components in Azotobacter vinelandii. Biochim Biophys Acta. 1974; 362(1):160-6. DOI: 10.1016/0304-4165(74)90037-3. View

KNIGHT Jr E, Hardy R . Isolation and characteristics of flavodoxin from nitrogen-fixing Clostridium pasteurianum. J Biol Chem. 1966; 241(12):2752-6. View

MASSEY V, Hemmerich P . A photochemical procedure for reduction of oxidation-reduction proteins employing deazariboflavin as catalyst. J Biol Chem. 1977; 252(16):5612-4. View

Yates M . Electron transport to nitrogenase in Azotobacter chroococcum: Azotobacter flavodoxin hydroquinone as an electron donor. FEBS Lett. 1972; 27(1):63-67. DOI: 10.1016/0014-5793(72)80410-1. View

Burnett R, Darling G, Kendall D, LeQuesne M, Mayhew S, Smith W . The structure of the oxidized form of clostridial flavodoxin at 1.9-A resolution. J Biol Chem. 1974; 249(14):4383-92. View

Dixon R, Cannon F, Kondorosi A . Construction of a P plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae. Nature. 1976; 260(5548):268-71. DOI: 10.1038/260268a0. View

Shah V, Davis L, Brill W . Nitrogenase. I. Repression and derepression of the iron-molybdenum and iron proteins of nitrogenase in Azotobacter vinelandii. Biochim Biophys Acta. 1972; 256(2):498-511. DOI: 10.1016/0005-2728(72)90078-3. View

10.

Cusanovich M, Edmondson D . The isolation and characterization of Rhodospirillum rubrum flavodoxin. Biochem Biophys Res Commun. 1971; 45(2):327-36. DOI: 10.1016/0006-291x(71)90822-9. View

11.

Orme-Johnson W, Hamilton W, Jones T, Tso M, BURRIS R, Shah V . Electron paramagnetic resonance of nitrogenase and nitrogenase components from Clostridium pasteurianum W5 and Azotobacter vinelandii OP. Proc Natl Acad Sci U S A. 1972; 69(11):3142-5. PMC: 389722. DOI: 10.1073/pnas.69.11.3142. View

12.

Hill S, Kavanagh E . Roles of nifF and nifJ gene products in electron transport to nitrogenase in Klebsiella pneumoniae. J Bacteriol. 1980; 141(2):470-5. PMC: 293649. DOI: 10.1128/jb.141.2.470-475.1980. View

13.

St John R, Johnston H, Seidman C, Garfinkel D, Gordon J, Shah V . Biochemistry and genetics of Klebsiella pneumoniae mutant strains unable to fix N2. J Bacteriol. 1975; 121(3):759-65. PMC: 246000. DOI: 10.1128/jb.121.3.759-765.1975. View

14.

Hinkson J . Azotobacter free-radical flavoprotein. Preparation and properties of the apoprotein. Biochemistry. 1968; 7(7):2666-72. DOI: 10.1021/bi00847a033. View

15.

Faeder E, Siegel L . A rapid micromethod for determination of FMN and FAD in mixtures. Anal Biochem. 1973; 53(1):332-6. DOI: 10.1016/0003-2697(73)90442-9. View

16.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

17.

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

18.

Roberts G, MacNeil T, MacNeil D, Brill W . Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae. J Bacteriol. 1978; 136(1):267-79. PMC: 218657. DOI: 10.1128/jb.136.1.267-279.1978. View

19.

Dubourdieu M, Le Gall J . Chemical study of two flavodoxins extracted from sulfate reducing bacteria. Biochem Biophys Res Commun. 1970; 38(5):965-72. DOI: 10.1016/0006-291x(70)90816-8. View