Biochemistry and Genetics of Klebsiella Pneumoniae Mutant Strains Unable to Fix N2

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1975 Mar 1

PMID 1090602

Citations 35

Authors

R T St John

H M Johnston

C Seidman

D Garfinkel

J K Gordon

V K Shah

W J Brill

Affiliations

Soon will be listed here.

Abstract

Selected mutant strains of Klebsiella pneumoniae that are unable to fix nitrogen have been characterized according to nitrogenase component activity as well as antigenic cross-reacting material. The lesions in these strains have been mapped by transduction, and the results indicate that there are at least five genes specifically responsible for nitrogen fixation in vivo. Besides genes that specify the structure of the two nitrogenase components, there is a gene for a factor that is required for component I activity and a gene that codes for a factor possibly involved in electron transport to component II. A mutation in another site does not allow the organism to produce either of the nitrogenase components. All of these genes are co-transducible with the gene that specifics the structure of histidinol dehydrogenase.

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References

Brill W, Westphal J, Stieghorst M, Davis L, Shah V . Detection of nitrogenase components and other nonheme iron proteins in polyacrylamide gels. Anal Biochem. 1974; 60(1):237-41. DOI: 10.1016/0003-2697(74)90149-3. View

DEMEREC M, Adelberg E, Clark A, Hartman P . A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966; 54(1):61-76. PMC: 1211113. DOI: 10.1093/genetics/54.1.61. View

Shah V, Davis L, Stieghorst M, Brill W . Mutant of Azotobacter vinelandii that hyperproduces nitrogenase component II. J Bacteriol. 1974; 117(2):917-9. PMC: 285593. DOI: 10.1128/jb.117.2.917-919.1974. View

Orme-Johnson W, Hamilton W, Jones T, Tso M, BURRIS R, Shah V . Electron paramagnetic resonance of nitrogenase and nitrogenase components from Clostridium pasteurianum W5 and Azotobacter vinelandii OP. Proc Natl Acad Sci U S A. 1972; 69(11):3142-5. PMC: 389722. DOI: 10.1073/pnas.69.11.3142. View

Nason A, Lee K, Pan S, Ketchum P, Lamberti A, Devries J . Invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a Neurospora mutant and a component of molybdenum-enzymes. Proc Natl Acad Sci U S A. 1971; 68(12):3242-6. PMC: 389631. DOI: 10.1073/pnas.68.12.3242. View

Streicher S, Gurney E, Valentine R . The nitrogen fixation genes. Nature. 1972; 239(5374):495-9. DOI: 10.1038/239495a0. View

Tubb R, POSTGATE J . Control of nitrogenase synthesis in Klebsiella pneumoniae. J Gen Microbiol. 1973; 79(1):103-17. DOI: 10.1099/00221287-79-1-103. View

Brill W, Steiner A, Shah V . Effect of molybdenum starvation and tungsten on the synthesis of nitrogenase components in Klebsiella pneumonia. J Bacteriol. 1974; 118(3):986-9. PMC: 246848. DOI: 10.1128/jb.118.3.986-989.1974. View

Wolf B, Newman A, Glaser D . On the origin and direction of replication of the Escherichia coli K12 chromosome. J Mol Biol. 1968; 32(3):611-29. DOI: 10.1016/0022-2836(68)90346-x. View

10.

Dixon R, POSTGATE J . Transfer of nitrogen-fixation genes by conjugation in Klebsiella pneumoniae. Nature. 1971; 234(5323):47-8. DOI: 10.1038/234047a0. View

11.

Fisher R, Brill W . Mutants of Azotobacter vinelandii unable to fix nitrogen. Biochim Biophys Acta. 1969; 184(1):99-105. DOI: 10.1016/0304-4165(69)90103-2. View

12.

Yoch D, PENGRA R . Effect of amino acids on the nitrogenase system of Klebsiella pneumoniae. J Bacteriol. 1966; 92(3):618-22. PMC: 276299. DOI: 10.1128/jb.92.3.618-622.1966. View

13.

Gordon J, Brill W . Mutants that produce nitrogenase in the presence of ammonia. Proc Natl Acad Sci U S A. 1972; 69(12):3501-3. PMC: 389805. DOI: 10.1073/pnas.69.12.3501. View

14.

Prival M, Brenchley J, MAGASANIK B . Glutamine synthetase and the regulation of histidase formation in Klebsiella aerogenes. J Biol Chem. 1973; 248(12):4334-44. View

15.

Tyler B, DeLeo A, MAGASANIK B . Activation of transcription of hut DNA by glutamine synthetase. Proc Natl Acad Sci U S A. 1974; 71(1):225-9. PMC: 387970. DOI: 10.1073/pnas.71.1.225. View

16.

Streicher S, Shanmugam K, Ausubel F, Morandi C, Goldberg R . Regulation of nitrogen fixation in Klebsiella pneumoniae: evidence for a role of glutamine synthetase as a regulator of nitrogenase synthesis. J Bacteriol. 1974; 120(2):815-21. PMC: 245843. DOI: 10.1128/jb.120.2.815-821.1974. View

17.

Nagatani H, Shah V, Brill W . Activation of inactive nitrogenase by acid-treated component I. J Bacteriol. 1974; 120(2):697-701. PMC: 245829. DOI: 10.1128/jb.120.2.697-701.1974. View

18.

Roth J . UGA nonsense mutations in Salmonella typhimurium. J Bacteriol. 1970; 102(2):467-75. PMC: 247572. DOI: 10.1128/jb.102.2.467-475.1970. View

19.

Shah V, Davis I, Gordon J, Orme-Johnson W, Brill W . Nitrogenase. 3. Nitrogenaseless mutants of Azotobacter vinelandii: activities, cross-reactions and EPR spectra. Biochim Biophys Acta. 1973; 292(1):246-55. DOI: 10.1016/0005-2728(73)90269-7. View

20.

Nagatani H, Brill W . Nitrogenase V. The effect of Mo, W and V on the synthesis of nitrogenase components in Azotobacter vinelandii. Biochim Biophys Acta. 1974; 362(1):160-6. DOI: 10.1016/0304-4165(74)90037-3. View