Characteristics of 5-hydroxytryptamine Transport in Pancreatic Islets

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1980 Apr 1

PMID 6991048

Citations 5

Authors

P Lindstrom

J Sehlin

I B Taljedal

Affiliations

Soon will be listed here.

Abstract

1 Transmembrane transport of 3H-labelled 5-hydroxytryptamine (5-HT) by isolated pancreatic islets of non-inbred ob/ob mice was studied. 2 5-HT was vigorously accumulated in a temperature-dependent way by the islet cells. 3 Studies of the concentration-dependence of [3H]-5-HT uptake revealed complex kinetics with one component being saturated at 1 to 3 microM 5-HT (apparent association constant 0.6 x 10(6) M(-1) and the other non-saturated up to 1 mM 5-HT. 4 The saturable uptake was inhibited by Na+-deficiency and metabolic poisoning with 2,4-dinitrophenol and antimycin A, whereas the non-saturable component was not affected. 5 Omission of K+, Ca2+ or Mg2+ did not affect the uptake rate. 6 It is concluded that 5-HT is taken up by pancreatic beta-cells by mechanisms very similar to those observed in thrombocytes and neurones.

Citing Articles

Amplification of exocytosis by Ca2+-induced Ca2+ release in INS-1 pancreatic beta cells.

Kang G, Holz G J Physiol. 2003; 546(Pt 1):175-89.

PMID: 12509487 PMC: 2342456. DOI: 10.1113/jphysiol.2002.029959.

Enhancement of [m-methoxy 3H]MDL100907 binding to 5HT2A receptors in cerebral cortex and brain stem of streptozotocin induced diabetic rats.

Jackson J, Paulose C Mol Cell Biochem. 1999; 199(1-2):81-5.

PMID: 10544955 DOI: 10.1023/a:1006938713276.

Further studies on 5-hydroxytryptamine transport in pancreatic islets and isolated beta-cells.

Lindstrom P Br J Pharmacol. 1981; 73(2):385-91.

PMID: 7016232 PMC: 2071649. DOI: 10.1111/j.1476-5381.1981.tb10433.x.

Opposite effects of 5-hydroxytryptophan and 5-hydroxytryptamine on the function of microdissected ob/ob-mouse pancreatic islets.

Lindstrom P, Sehlin J Diabetologia. 1983; 24(1):52-7.

PMID: 6337907 DOI: 10.1007/BF00275948.

5-Hydroxytryptamine transport in cells and secretory granules from a transplantable rat insulinoma.

Hutton J, Peshavaria M, Tooke N Biochem J. 1983; 210(3):803-10.

PMID: 6307272 PMC: 1154293. DOI: 10.1042/bj2100803.

References

Hellman B . Studies in obese-hyperglycemic mice. Ann N Y Acad Sci. 1965; 131(1):541-58. DOI: 10.1111/j.1749-6632.1965.tb34819.x. View

BOGDANSKI D, Brodie B . Role of sodium and potassium ions in storage of norepinephrine by sympathetic nerve endings. Life Sci. 1966; 5(17):1563-9. DOI: 10.1016/0024-3205(66)91025-3. View

Blackburn K, French P, MERRILLS R . 5-hydroxytryptamine uptake by rat brain in vitro. Life Sci. 1967; 6(15):1653-63. DOI: 10.1016/0024-3205(67)90176-2. View

Pletscher A . Metabolism, transfer and storage of 5-hydroxytryptamine in blood platelets. Br J Pharmacol Chemother. 1968; 32(1):1-16. PMC: 1570304. DOI: 10.1111/j.1476-5381.1968.tb00423.x. View

ABRAMS W, Solomon H . The human platelet as a pharmacologic model for the adrenergic neuron. The uptake and release of norepinephrine. Clin Pharmacol Ther. 1969; 10(5):702-9. DOI: 10.1002/cpt1969105702. View

Sneddon J . Sodium-dependent accumulation of 5-hydroxytryptamine by rat blood platelets. Br J Pharmacol. 1969; 37(3):680-8. PMC: 1703717. DOI: 10.1111/j.1476-5381.1969.tb08506.x. View

Feldman J, Lebovitz H . Specificity of serotonin inhibition of insulin release from golden hamster pancreas. Diabetes. 1970; 19(7):475-9. DOI: 10.2337/diab.19.7.475. View

Shaskan E, Snyder S . Kinetics of serotonin accumulation into slices from rat brain: relationship to catecholamine uptake. J Pharmacol Exp Ther. 1970; 175(2):404-18. View

Hellman B, Sehlin J, Taljedal I . Transport of -aminoisobutyric acid in mammalian pancretic -cells. Diabetologia. 1971; 7(4):256-65. DOI: 10.1007/BF01211878. View

10.

Hellman B, Sehlin J, Taljedal I . Evidence for mediated transport of glucose in mammalian pancreatic -cells. Biochim Biophys Acta. 1971; 241(1):147-54. DOI: 10.1016/0005-2736(71)90312-9. View

11.

EKHOLM R, Ericson L, Lundquist I . Monoamines in the pancreatic islets of the mouse. Subcellular localization of 5-hydroxytryptamine by electron microscopic autoradiography. Diabetologia. 1971; 7(5):339-48. DOI: 10.1007/BF01219468. View

12.

Lernmark A . The significance of 5-hydroxytryptamine for insulin secretion in the mouse. Horm Metab Res. 1971; 3(5):305-9. DOI: 10.1055/s-0028-1094131. View

13.

Hellman B, Lernmark A, Sehlin J, Taljedal I . Transport and storage of 5-hydroxytryptamine in pancreatic -cells. Biochem Pharmacol. 1972; 21(5):695-706. DOI: 10.1016/0006-2952(72)90062-7. View

14.

Lebovitz H, Feldman J . Pancreatic biogenic amines and insulin secretion in health and disease. Fed Proc. 1973; 32(7):1797-802. View

15.

Gylfe E, Hellman B, Sehlin J, Taljedal I . Amino acid conversion into 5-hydroxytryptamine in pancreatic beta-cells. Endocrinology. 1973; 93(4):932-7. DOI: 10.1210/endo-93-4-932. View

16.

Sneddon J . Blood platelets as a model for monoamine-containing neurones. Prog Neurobiol. 1973; 1(2):151-98. DOI: 10.1016/0301-0082(73)90019-1. View

17.

Wilson J, Downs Jr R, Feldman J, Lebovitz H . Beta cell monoamines: further evidence for their role in modulating insulin secretion. Am J Physiol. 1974; 227(2):305-12. DOI: 10.1152/ajplegacy.1974.227.2.305. View

18.

Gagliardino J, Nierle C, Pfeiffer E . The effect of serotonin on in vitro insulin secretion and biosynthesis in mice. Diabetologia. 1974; 10(5):411-4. DOI: 10.1007/BF01221630. View

19.

Feldman J, Chapman B . Characterization of pancreatic islet monoamine oxidase. Metabolism. 1975; 24(5):581-8. DOI: 10.1016/0026-0495(75)90138-9. View

20.

Ross S, RENYI A . Tricyclic antidepressant agents. I. Comparison of the inhibition of the uptake of 3-H-noradrenaline and 14-C-5-hydroxytryptamine in slices and crude synaptosome preparations of the midbrain-hypothalamus region of the rat brain. Acta Pharmacol Toxicol (Copenh). 1975; 36(Suppl 5):382-94. DOI: 10.1111/j.1600-0773.1975.tb00806.x. View