Three Distinct Antigens Associated with Human T-lymphocyte-mediated Cytolysis: LFA-1, LFA-2, and LFA-3

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Dec 1

PMID 6984191

Citations 223

Authors

F Sanchez-Madrid

A M Krensky

C F Ware

E Robbins

J L Strominger

S J Burakoff

T A Springer

Affiliations

Soon will be listed here.

Abstract

Monoclonal antibodies were prepared to anti-HLA-DR cytolytic T lymphocytes (CTLs) and screened for inhibition of CTL-mediated killing. Binding of monoclonal antibodies to four types of molecules, LFA-1, LFA-2, LFA-3, and HLA-DR, inhibited killing, suggesting that these molecules participate in the CTL-target cell interaction. The antigens were characterized by immunoprecipitation, crosslinking, NaDodSO4/polyacrylamide gel electrophoresis, and immunofluorescence flow cytometry. The LFA-1 antigen contains alpha and beta polypeptide chains of Mr 177,000 and 95,000 that are noncovalently associated in an alpha 1 beta 1 structure. It is present on both B and T lymphocytes and marks subpopulations that differ in quantitative expression. Human LFA-1 appears to be the homologue of mouse LFA-1. Human LFA-2 is of Mr 49,000 with a minor component of Mr 36,000. It is expressed on CTL lines but not on a B-cell line and in peripheral blood preferentially on T lymphocytes. Human LFA-3 is of Mr 60,000 and is expressed on both B and T lymphocytes.

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References

Golstein P . Divalent cation requirements as a tool for the study of cell-mediated cytotoxocity systems. Adv Exp Med Biol. 1976; 66:465-70. DOI: 10.1007/978-1-4613-4355-4_71. View

Martz E . Mechanism of specific tumor-cell lysis by alloimmune T lymphocytes: resolution and characterization of discrete steps in the cellular interaction. Contemp Top Immunobiol. 1977; 7:301-61. DOI: 10.1007/978-1-4684-3054-7_9. View

Laskey R, Mills A . Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Lett. 1977; 82(2):314-6. DOI: 10.1016/0014-5793(77)80609-1. View

Springer T, Galfre G, Secher D, Milstein C . Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979; 9(4):301-6. DOI: 10.1002/eji.1830090410. View

Berke G . Interaction of cytotoxic T lymphocytes and target cells. Prog Allergy. 1980; 27:69-133. View

Reinherz E, Hussey R, Schlossman S . A monoclonal antibody blocking human T cell function. Eur J Immunol. 1980; 10(10):758-62. DOI: 10.1002/eji.1830101006. View

Reiss C, Hemler M, Englehard V, Mier J, Strominger J, Burakoff S . Development and characterization of allospecific long-term human cytolytic T-cell lines. Proc Natl Acad Sci U S A. 1980; 77(9):5432-6. PMC: 350073. DOI: 10.1073/pnas.77.9.5432. View

Chang T, Kung P, Gingras S, Goldstein G . Does OKT3 monoclonal antibody react with an antigen-recognition structure on human T cells?. Proc Natl Acad Sci U S A. 1981; 78(3):1805-8. PMC: 319223. DOI: 10.1073/pnas.78.3.1805. View

Ledbetter J, Evans R, Lipinski M, Cunningham-Rundles C, GOOD R, Herzenberg L . Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-23. PMC: 2186068. DOI: 10.1084/jem.153.2.310. View

10.

Evans R, Wall D, Platsoucas C, Siegal F, Fikrig S, Testa C . Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-8. PMC: 319090. DOI: 10.1073/pnas.78.1.544. View

11.

Davignon D, Martz E, Reynolds T, Kurzinger K, Springer T . Monoclonal antibody to a novel lymphocyte function-associated antigen (LFA-1): mechanism of blockade of T lymphocyte-mediated killing and effects on other T and B lymphocyte functions. J Immunol. 1981; 127(2):590-5. View

12.

Kurzinger K, Reynolds T, Germain R, Davignon D, Martz E, Springer T . A novel lymphocyte function-associated antigen (LFA-1): cellular distribution, quantitative expression, and structure. J Immunol. 1981; 127(2):596-602. View

13.

Platsoucas C, GOOD R . Inhibition of specific cell-mediated cytotoxicity by monoclonal antibodies to human T cell antigens. Proc Natl Acad Sci U S A. 1981; 78(7):4500-4. PMC: 319819. DOI: 10.1073/pnas.78.7.4500. View

14.

Galfre G, Milstein C . Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 1981; 73(Pt B):3-46. DOI: 10.1016/0076-6879(81)73054-4. View

15.

Reinherz E, Hussey R, Fitzgerald K, Snow P, Terhorst C, Schlossman S . Antibody directed at a surface structure inhibits cytolytic but not suppressor function of human T lymphocytes. Nature. 1981; 294(5837):168-70. DOI: 10.1038/294168a0. View

16.

Kurzinger K, Ho M, Springer T . Structural homology of a macrophage differentiation antigen and an antigen involved in T-cell-mediated killing. Nature. 1982; 296(5858):668-70. DOI: 10.1038/296668a0. View

17.

Krensky A, Reiss C, Mier J, Strominger J, Burakoff S . Long-term human cytolytic T-cell lines allospecific for HLA-DR6 antigen are OKT4+. Proc Natl Acad Sci U S A. 1982; 79(7):2365-9. PMC: 346194. DOI: 10.1073/pnas.79.7.2365. View

18.

Malissen B, Mawas C, Rebai N . Inhibition of human T cell mediated cytolysis by monoclonal antibodies to effector cell surface structures. Adv Exp Med Biol. 1982; 146:563-73. DOI: 10.1007/978-1-4684-8959-0_35. View

19.

Kurzinger K, Springer T . Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor. J Biol Chem. 1982; 257(20):12412-8. View

20.

Krensky A, Clayberger C, Reiss C, Strominger J, Burakoff S . Specificity of OKT4+ cytotoxic T lymphocyte clones. J Immunol. 1982; 129(5):2001-3. View