Human B Cell Differentiation. II. Pokeweed Mitogen-responsive B Cells Belong to a Surface Immunoglobulin D-negative Subpopulation

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1982 May 1

PMID 6978377

Citations 44

Authors

T Kuritani

M D Cooper

Affiliations

Soon will be listed here.

Abstract

Surface immunoglobulin D (IgD)-positive lymphocytes precoated with monoclonal anti-delta antibody were selectively removed from blood mononuclear cell preparations by "panning" and by fluorescence-activated cell sorter. The depletion of sIgD+ cells did not significantly affect plasma cell responses to pokeweed mitogen PWM). PWM-responsive B cells lacking sIgD and mouse erythrocyte receptors preferentially sedimented in lower density fractions of a discontinuous Percoll gradient, and sIgD-negative B cells were found to have a larger mean diameter than IgD-positive cells. We conclude that PWM-responsive B cells represent a distinct subpopulation of relatively large cells that have ceased to express receptors for mouse erythrocytes and surface IgD.

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