Stimulation of Chronic Lymphatic Leukaemia Cells by Pokeweed Mitogen After Treatment with Neuraminidase-galactose Oxidase

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1981 Apr 1

PMID 6973427

Citations 1

Authors

M N Zafar

S Pittman

M Cherchi

D Catovsky

Affiliations

Soon will be listed here.

Abstract

CLL lymphocytes gave a low response upon stimulation with PHA or PWM in 3-day cultures. However, after treatment with neuraminidase-galactose oxidase (NGO), in the presence of PWM, CLL lymphocytes transformed into blasts and incorporated 3H-thymidine in 3-day cultures. This response of CLL lymphocytes was similar to that given by normal lymphocytes to PWM in 3-day cultures. The best stimulation of CLL lymphocytes was achieved when conditioned medium (CM) from normal T lymphocytes was present in PWM cultures. Purified B lymphocytes from CLL (T lymphocytes and monocytes removed) did not respond to PHA or PWM. However, after NGO treatment these cells were stimulated by PWM, but only in the presence of CM. PHA failed to stimulate NGO-treated CLL lymphocytes or purified B lymphocytes. This study shows that CLL lymphocytes, which usually fail to respond to mitogens, can be stimulated by PWM to proliferate after treatment with neuraminidase-galactose oxidase (NGO). This technique of B cell stimulation has been found useful in cytogenetic studies of B cell proliferative disorders.

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PMID: 8376314 DOI: 10.1007/BF02634550.

References

Catovsky D, TRIPP E, Hoffbrand A . Response to phytohaemagglutinin and pokeweed mitogen in chronic lymphocytic leukaemia. Lancet. 1972; 1(7754):794-5. View

Jondal M, Holm G, Wigzell H . Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136(2):207-15. PMC: 2139198. DOI: 10.1084/jem.136.2.207. View

Novogrodsky A, KATCHALSKI E . Induction of lymphocyte transformation by sequential treatment with neuraminidase and galactose oxidase. Proc Natl Acad Sci U S A. 1973; 70(6):1824-7. PMC: 433605. DOI: 10.1073/pnas.70.6.1824. View

Greaves M, Janossy G, Doenhoff M . Selective triggering of human T and B lymphocytes in vitro by polyclonal mitogens. J Exp Med. 1974; 140(1):1-18. PMC: 2139703. DOI: 10.1084/jem.140.1.1. View

Klemm W . Dissociation of EEG and behavioural effects of ethanol provide evidence for a noncholinergic basis of intoxication. Nature. 1974; 251(5472):234-6. DOI: 10.1038/251234a0. View

Novogrodsky A . Selective activation of mouse T and B lymphocytes by periodate, galactose oxidase and soybean agglutinin. Eur J Immunol. 1974; 4(10):646-8. DOI: 10.1002/eji.1830041003. View

Catovsky D, Cherchi M, Okos A, Hegde U, GALTON D . Mouse red-cell rosettes in B-lymphoproliferative disorders. Br J Haematol. 1976; 33(2):173-7. DOI: 10.1111/j.1365-2141.1976.tb03528.x. View

Godal T, Henriksen A, Iversen J, Landaas T, Lindmo T . Altered membrane-associated functions in chronic lymphocytic leukemia cells. Int J Cancer. 1978; 21(5):561-9. DOI: 10.1002/ijc.2910210505. View

Han T, Dadey B . In vitro functional studies of mononuclear cells in patients with CLL: evidence for functionally normal T lymphocytes and monocytes and abnormal B lymphocytes. Cancer. 1979; 43(1):109-17. DOI: 10.1002/1097-0142(197901)43:1<109::aid-cncr2820430117>3.0.co;2-g. View

10.

Wiley J, Kraft N, Cooper I . The binding of ouabain to normal and chronic lymphocytic leukemic lymphocytes. Blood. 1979; 54(5):994-1000. View

11.

Robert K . PHA-induced soluble factor(s) can activate B-cells from patients with chronic lymphatic leukaemia. Clin Exp Immunol. 1979; 37(3):517-22. PMC: 1537779. View

12.

Johnsen H, Madsen M . Lymphocyte subpopulations in man: B-cell stimulation induced by pokeweed mitogen and irradiated T cells. Scand J Immunol. 1979; 10(3):241-9. DOI: 10.1111/j.1365-3083.1979.tb01346.x. View

13.

Sakano T . Enhancement of T-cell response to PWM by neuraminidase-treated non-T cells. Immunology. 1980; 39(2):203-7. PMC: 1457958. View

14.

Bernard C, Geraldes A, Boiron M . EFFECTS OF PHYTOHAEMAGGLUTININ ON BLOOD-CULTURES OF CHRONIC LYMPHOCYTIC LEUKAEMIAS. Lancet. 1964; 1(7334):667-8. DOI: 10.1016/s0140-6736(64)91488-6. View