Human Erythroid Burst-forming Units. Growth in Vitro is Dependent on Monocytes, but Not T Lymphocytes

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1981 Mar 1

PMID 6970752

Citations 17

Authors

K S Zuckerman

Affiliations

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Abstract

The roles of monocytes and T lymphocytes in the regulation of human peripheral blood erythroid burst-forming units (BFU-E) were studied in erythropoietin-containing plasma clot cultures of subpopulations of human blood mononuclear cells. BFU-E growth was decreased significantly after depletion of monocytes alone (mean 11% of expected, range 0 to 42% of expected) or depletion of both monocytes and T cells (mean 6.5% of expected, range 0.5 to 12% of expected) from mononuclear cells. T cell depletion did not impair BFU-E growth in vitro. Using 10(5) monocyte- and T lymphocyte-depleted mononuclear cells as target cells (less than 1% monocytes, less than 5% T cells), BFU-E growth was restored to 40% of expected by addition of 10(4) monocytes, and to 96% of expected by 10(5) monocytes alone. Addition of as many as 2 X 10(5) T cells but no monocytes resulted in stimulation to only 34% of expected BFU-E growth. Addition of 2 X 10(4) T cells, which alone did not affect BFU-E growth, could augment significantly the stimulatory effect of 5-20 X 10(3) monocytes on BFU-E growth. Thus, monocytes alone appear to be capable of stimulating BFU-E growth in vitro in the presence of erythropoietin. T cells also may make small quantities of BFU-E stimulators. However, it seems more likely that the most important role of T lymphocytes in BFU-E regulation in vitro is a result of interactions with monocytes and augmentation of monocyte production of stimulators of BFU-E growth.

Citing Articles

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CD3(+) and/or CD14(+) depletion from cord blood mononuclear cells before ex vivo expansion culture improves total nucleated cell and CD34(+) cell yields.

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References

Rocklin R, Winston C, David J . Activation of human blood monocytes by products of sensitized lymphocytes. J Clin Invest. 1974; 53(2):559-64. PMC: 301499. DOI: 10.1172/JCI107590. View

Schaller J, Davis S, Ching Y, Lagunoff D, Williams C, Wedgwood R . Hypergammaglobulinaemia, antibody deficiency, autoimmune haemolytic anaemia, and nephritis in an infant with a familial lymphopenic immune defect. Lancet. 1966; 2(7468):825-9. DOI: 10.1016/s0140-6736(66)92255-0. View

Boyum A . Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968; 97:77-89. View

Chervenick P, LoBuglio A . Human blood monocytes: stimulators of granulocyte and mononuclear colony formation in vitro. Science. 1972; 178(4057):164-6. DOI: 10.1126/science.178.4057.164. View

Golde D, Cline M . Identification of the colony-stimulating cell in human peripheral blood. J Clin Invest. 1972; 51(11):2981-3. PMC: 292450. DOI: 10.1172/JCI107124. View

Golde D, FINLEY T, Cline M . Production of colony-stimulating factor by human macrophages. Lancet. 1972; 2(7792):1397-9. DOI: 10.1016/s0140-6736(72)92966-2. View

Mendes N, Tolnai M, Silveira N, Gilbertsen R, METZGAR R . Technical aspects of the rosette tests used to detect human complement receptor (B) and sheep erythrocyte-binding (T) lymphocytes. J Immunol. 1973; 111(3):860-7. View

Ruscetti F, Chervenick P . Release of colony-stimulating factor from monocytes by endotoxin and polyinosinic-polycytidylic acid. J Lab Clin Med. 1974; 83(1):64-72. View

Cline M, Golde D . Production of colony-stimulating activity by human lymphocytes. Nature. 1974; 248(5450):703-4. DOI: 10.1038/248703a0. View

10.

Tepperman A, Curtis J, McCulloch E . Erythropietic colonies in cultures of human marrow. Blood. 1974; 44(5):659-69. View

11.

Einstein L, Schneeberger E, Colten H . Synthesis of the second component of complement by long-term primary cultures of human monocytes. J Exp Med. 1976; 143(1):114-26. PMC: 2190106. DOI: 10.1084/jem.143.1.114. View

12.

Mookerjee B . Functional characteristics of monocytes. 1. Essential role in the transformational response of human blood lymphocytes to phytomitogens. Transplantation. 1977; 23(1):22-8. View

13.

Tucker S, PIERRE R, Jordon R . Rapid identification of monocytes in a mixed mononuclear cell preparation. J Immunol Methods. 1977; 14(3-4):267-9. DOI: 10.1016/0022-1759(77)90137-5. View

14.

Hara H, Ogawa M . Erythropoietic precursors in mice under erythropoietic stimulation and suppression. Exp Hematol. 1977; 5(2):141-8. View

15.

Aye M . Erythroid colony formation in cultures of human marrow: effect of leukocyte conditioned medium. J Cell Physiol. 1977; 91(1):69-77. DOI: 10.1002/jcp.1040910108. View

16.

Gregory C, Eaves A . Human marrow cells capable of erythropoietic differentiation in vitro: definition of three erythroid colony responses. Blood. 1977; 49(6):855-64. View

17.

Iscove N . The role of erythropoietin in regulation of population size and cell cycling of early and late erythroid precursors in mouse bone marrow. Cell Tissue Kinet. 1977; 10(4):323-34. DOI: 10.1111/j.1365-2184.1977.tb00300.x. View

18.

Nathan D, Chess L, Hillman D, Clarke B, Breard J, Merler E . Human erythroid burst-forming unit: T-cell requirement for proliferation in vitro. J Exp Med. 1978; 147(2):324-39. PMC: 2184491. DOI: 10.1084/jem.147.2.324. View

19.

Zuckerman K, Sullivan R, Quesenberry P . Effects of actinomycin D in vivo on murine erythroid stem cells. Blood. 1978; 51(5):957-69. View

20.

Golde D, Quan S, Cline M . Human T lymphocyte cell line producing colony-stimulating activity. Blood. 1978; 52(5):1068-72. View