The Protein Composition of Mycoplasma Capricolum

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1982 Jan 1

PMID 6960228

Citations 13

Authors

Y Kawauchi

A Muto

S Osawa

Affiliations

Soon will be listed here.

Abstract

The whole cell proteins and the ribosomal proteins of Mycoplasma capricolum ATCC 27343 have been analyzed by two-dimensional polyacrylamide gel electrophoresis. The M. capricolum cell is relatively rich in basic proteins. The number of total protein spots detected was approximately 355, which is less than one-third of that of Escherichia coli or Bacillus subtilis. In contrast, the number (30 and 20 protein species have been found to be present in the 50S and 30S ribosomal subunits, respectively) and the size of the ribosomal proteins in the M. capricolum do not seem to be significantly different from those of typical eubacteria.

Citing Articles

Complexity of the Mycoplasma fermentans M64 genome and metabolic essentiality and diversity among mycoplasmas.

Shu H, Liu T, Chan H, Liu Y, Wu K, Shu H PLoS One. 2012; 7(4):e32940.

PMID: 22509252 PMC: 3317919. DOI: 10.1371/journal.pone.0032940.

RNase P RNA of Mycoplasma capricolum.

Ushida C, Izawa D, Muto A Mol Biol Rep. 1995; 22(2-3):125-9.

PMID: 8901498 DOI: 10.1007/BF00988716.

A survey of the Mycoplasma genitalium genome by using random sequencing.

Peterson S, Hu P, Bott K, HUTCHISON 3rd C J Bacteriol. 1993; 175(24):7918-30.

PMID: 8253680 PMC: 206970. DOI: 10.1128/jb.175.24.7918-7930.1993.

A small RNA of Mycoplasma capricolum that resembles eukaryotic U6 small nuclear RNA.

Ushida C, Muto A Nucleic Acids Res. 1993; 21(11):2649-53.

PMID: 7687343 PMC: 309594. DOI: 10.1093/nar/21.11.2649.

Nucleotide sequence of the rrnB 16S ribosomal RNA gene from Mycoplasma capricolum.

Iwami M, Muto A, Yamao F, Osawa S Mol Gen Genet. 1984; 196(2):317-22.

PMID: 6208458 DOI: 10.1007/BF00328065.

References

Wallace D, Morowitz H . Genome size and evolution. Chromosoma. 1973; 40(2):121-6. DOI: 10.1007/BF00321457. View

Sawada M, Osawa S, Kobayashi H, Hori H, Muto A . The number of ribosomal RNA genes in Mycoplasma capricolum. Mol Gen Genet. 1981; 182(3):502-4. DOI: 10.1007/BF00293942. View

Isono K, Isono S . Lack of ribosomal protein S1 in Bacillus stearothermophilus. Proc Natl Acad Sci U S A. 1976; 73(3):767-70. PMC: 335999. DOI: 10.1073/pnas.73.3.767. View

Razin S . The mycoplasmas. Microbiol Rev. 1978; 42(2):414-70. PMC: 281436. DOI: 10.1128/mr.42.2.414-470.1978. View

Johnson J, Horowitz J . Characterization of ribosomes and RNAs from Mycoplasma hominis. Biochim Biophys Acta. 1971; 247(2):262-79. DOI: 10.1016/0005-2787(71)90675-7. View

OFarrell P, Goodman H, Ofarrell P . High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977; 12(4):1133-41. DOI: 10.1016/0092-8674(77)90176-3. View

Hardy S, Kurland C, Voynow P, Mora G . The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969; 8(7):2897-905. DOI: 10.1021/bi00835a031. View

Hori H, Sawada M, Osawa S, Murao K, Ishikura H . The nucleotide sequence of 5S rRNA from Mycoplasma capricolum. Nucleic Acids Res. 1981; 9(20):5407-10. PMC: 327528. DOI: 10.1093/nar/9.20.5407. View

Mets L, Bogorad L . Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins. Anal Biochem. 1974; 57(1):200-10. DOI: 10.1016/0003-2697(74)90065-7. View

10.

Higo K, Otaka E, Osawa S . Purification and characterization of 30S ribosomal proteins from Bacillus subtilis: correlation to Escherichia coli 30S proteins. Mol Gen Genet. 1982; 185(2):239-44. DOI: 10.1007/BF00330792. View

11.

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

12.

Osawa S, Tokui A, Saito H . Mapping by interspecies transformation experiments of several ribosomal protein genes near the replication origin of Bacillus subtilis chromosome. Mol Gen Genet. 1978; 164(2):113-29. DOI: 10.1007/BF00267376. View

13.

Kaltschmidt E, WITTMANN H . Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970; 36(2):401-12. DOI: 10.1016/0003-2697(70)90376-3. View

14.

Ryan J, Morowitz H . Partial purification of native rRNA and tRNA cistrons from mycoplasma sp. (Kid). Proc Natl Acad Sci U S A. 1969; 63(4):1282-9. PMC: 223462. DOI: 10.1073/pnas.63.4.1282. View