DNA Sequence Associated with Chromosome Translocations in Mouse Plasmacytomas

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Nov 1

PMID 6959142

Citations 18

Authors

L J Harris

P DEustachio

F H Ruddle

K B Marcu

Affiliations

Soon will be listed here.

Abstract

A DNA sequence that generates aberrantly rearranged immunoglobulin heavy chain constant region genes in murine plasmacytomas is shown to participate in a chromosome translocation. We have previously termed this DNA sequence NIARD for non-immunoglobulin-associated rearranging DNA. NIARD rearrangements were found frequently in murine plasmacytomas but were not detected in normal lymphocytes. These rearrangements occasionally involve the switch region of the C alpha gene. In this study, DNA samples obtained from mouse-Chinese hamster somatic cell hybrid lines were digested with various restriction endonucleases and analyzed by the Southern transfer technique with a NIARD hybridization probe. These experiments show that NIARD resides on chromosome 15 in the mouse germ line. Since NIARD is found adjacent to the C alpha gene (located on chromosome 12) in some plasmacytomas, it is apparent that a translocation involving these two chromosomes has occurred. We have proposed a rcpT(12;15) model to explain our data. The implications of NIARD rearrangements for malignant transformation are discussed.

Citing Articles

Fusion of DNA region to murine immunoglobulin heavy chain locus corresponds to plasmacytoma-associated chromosome translocation.

Cory S, Adams J, Gerondakis S, Miller J, Gamble J, Wiener F EMBO J. 1983; 2(2):213-6.

PMID: 11894928 PMC: 555115. DOI: 10.1002/j.1460-2075.1983.tb01407.x.

Molecular cloning of translocations involving chromosome 15 and the immunoglobulin C alpha gene from chromosome 12 in two murine plasmacytomas.

Calame K, Kim S, Lalley P, Hill R, Davis M, Hood L Proc Natl Acad Sci U S A. 1982; 79(22):6994-8.

PMID: 6960358 PMC: 347261. DOI: 10.1073/pnas.79.22.6994.

Oncogenic genes and human malignancy.

Lebowitz P Yale J Biol Med. 1983; 56(2):121-9.

PMID: 6636835 PMC: 2589700.

The William Allan Memorial Award address: Reverse genetics and beyond.

Ruddle F Am J Hum Genet. 1984; 36(5):944-53.

PMID: 6594045 PMC: 1684509.

Cellular myc oncogene is altered by chromosome translocation to an immunoglobulin locus in murine plasmacytomas and is rearranged similarly in human Burkitt lymphomas.

Adams J, Gerondakis S, Webb E, Corcoran L, Cory S Proc Natl Acad Sci U S A. 1983; 80(7):1982-6.

PMID: 6572957 PMC: 393736. DOI: 10.1073/pnas.80.7.1982.

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