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Comparison of in Situ and Peripheral Host Immunity to Syngeneic Tumours Employing the Multicellular Spheroid Model

Overview
Journal Br J Cancer Suppl
Specialty Oncology
Date 1980 Apr 1
PMID 6932915
Citations 7
Authors
E M Lord
Affiliations
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Abstract

The multicellular tumour spheroid was used as a model system to assess the in situ host immune response to the EMT6/Ro mammary tumour in syngeneic BALB/cKa mice. In sensitized mice the spheroids were rapidly infiltrated by host cells including macrophages, lymphocytes and granulocytes. Tumour cell killing was evident within 1 day and resulted in the eventual complete destruction of the spheroids. Host cells within the spheroids had a greater cytolytic capacity than the surrounding peritoneal cells and virtually no cytolytic activity was detectable in cells from the spleen. A similar discrepancy between in situ and peripheral immunity was found in mice bearing solid EMT6/Ro tumours.

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