Specific (EMT6) and Non-specific (WEHI-164) Cytolytic Activity by Host Cells Infiltrating Tumour Spheroids

Overview

Journal Br J Cancer

Specialty Oncology

Date 1987 Feb 1

PMID 3493024

Citations 5

Authors

K M Wilson

E M Lord

Affiliations

Soon will be listed here.

Abstract

The development of a serum-free, low-protein culture medium has allowed the detection of tumour-specific cytolytic cells in EMT6 immunized mice bearing EMT6 multicellular tumour spheroids. Spheroid associated (SAC) and peritoneal (PC) effector cells were specific to EMT6 as the target cell, not killing line 1, P815 or RIF-1. The natural killer (NK) cell sensitive target YAC-1 was also not lysed by SAC or PC, indicating undetectable levels of NK cells present within infiltrated spheroids. In contrast, high levels of cytolytic activity were present in SAC, PC and spleen cells against WEHI-164, a line sensitive to natural cytotoxic (NC) and macrophage mediated killing. The EMT6 specific activity was mediated by Thyl+, Lyt2+ cells. The anti-WEHI-164 effector cell population was Thyl-, Lyt2-. The WEHI-164 killer cells were present in SAC and PC from unimmunized mice while the EMT6 specific effector cells were not. After separation of SAC and PC by size using centrifugal elutriation, anti-EMT6 activity was present only in the lymphocyte fraction while anti-WEHI-164 activity was enriched in the macrophage fraction.

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References

Sutherland R, McCredie J, Inch W . Growth of multicell spheroids in tissue culture as a model of nodular carcinomas. J Natl Cancer Inst. 1971; 46(1):113-20. View

Dunn T, Potter M . A transplantable mast-cell neoplasm in the mouse. J Natl Cancer Inst. 1957; 18(4):587-601. View

Cikes M, Friberg Jr S, Klein G . Progressive loss of H-2 antigens with concomitant increase of cell-surface antigen(s) determined by Moloney leukemia virus in cultured murine lymphomas. J Natl Cancer Inst. 1973; 50(2):347-62. DOI: 10.1093/jnci/50.2.347. View

Rollinghoff M, Warner N . Specificity of in vivo tumor rejection assessed by mixing immune spleen cells with target and unrelated tumor cells. Proc Soc Exp Biol Med. 1973; 144(3):813-8. DOI: 10.3181/00379727-144-37688. View

Yuhas J, Pazmino N, Proctor J, Toya R . A direct relationship between immune competence and the subcutaneous growth rate of a malignant murine lung tumor. Cancer Res. 1974; 34(4):722-8. View

DeLustro F, Haskill J . In situ cytotoxic T cells in a methylcholanthrene-induced tumor. J Immunol. 1978; 121(3):1007-9. View

Golstein P, Luciani M, Wagner H, Rollinghoff M . Mouse T cell-mediated cytolysis specifically triggered by cytophilic xenogeneic serum determinants: a caveat for the interpretation of experiments done under "syngeneic" conditions. J Immunol. 1978; 121(6):2533-8. View

Lord E, Penney D, Sutherland R, COOPER Jr R . Morphological and functional characteristics of cells infiltrating and destroying tumor multicellular spheroids in vivo. Virchows Arch B Cell Pathol Incl Mol Pathol. 1979; 31(2):103-16. DOI: 10.1007/BF02889928. View

Twentyman P, Brown J, Gray J, Franko A, Scoles M, Kallman R . A new mouse tumor model system (RIF-1) for comparison of end-point studies. J Natl Cancer Inst. 1980; 64(3):595-604. View

10.

MacDonald H, Sordat B . The multicellular tumor spheroid: a quantitative model for studies of in situ immunity. Contemp Top Immunobiol. 1980; 10:317-42. DOI: 10.1007/978-1-4684-3677-8_15. View

11.

Raulet D, Gottlieb P, Bevan M . Fractionation of lymphocyte populations with monoclonal antibodies specific for LYT-2.2 and LYT-3.1. J Immunol. 1980; 125(3):1136-43. View

12.

Lord E . Comparison of in situ and peripheral host immunity to syngeneic tumours employing the multicellular spheroid model. Br J Cancer Suppl. 1980; 4:123-7. PMC: 2149202. View

13.

Totterman T, Parthenais E, Hayry P, Timonen T, Saksela E . Cytological and functional analysis of inflammatory infiltrates in human malignant tumors. III. Further functional investigations using cultured autochthonous tumor cell lines and freeze-thawed infiltrating inflammatory cells. Cell Immunol. 1980; 55(1):219-26. DOI: 10.1016/0008-8749(80)90153-7. View

14.

Eremin O, Coombs R, Ashby J . Lymphocytes infiltrating human breast cancers lack K-cell activity and show low levels of NK-cell activity. Br J Cancer. 1981; 44(2):166-76. PMC: 2010737. DOI: 10.1038/bjc.1981.167. View

15.

Lattime E, Pecoraro G, Stutman O . Natural cytotoxic cells against solid tumors in mice. IV. Natural cytotoxic (NC) cells are not activated natural killer (NK) cells. Int J Cancer. 1982; 30(4):471-7. DOI: 10.1002/ijc.2910300414. View

16.

Lattime E, Pecoraro G, Cuttito M, Stutman O . Murine non-lymphoid tumors are lysed by a combination of NK and NC cells. Int J Cancer. 1983; 32(4):523-8. DOI: 10.1002/ijc.2910320421. View

17.

Lord E, Keng P . Methods for using centrifugal elutriation to separate malignant and lymphoid cell populations. J Immunol Methods. 1984; 68(1-2):147-55. DOI: 10.1016/0022-1759(84)90145-5. View

18.

Lord E, Burkhardt G . Assessment of in situ host immunity to syngeneic tumors utilizing the multicellular spheroid model. Cell Immunol. 1984; 85(2):340-50. DOI: 10.1016/0008-8749(84)90248-x. View

19.

Ferry B, Flannery G, Robins R, Lawry J, Baldwin R . Phenotype of cytotoxic effector cells infiltrating a transplanted, chemically induced rat sarcoma. Immunology. 1984; 53(2):243-50. PMC: 1454825. View

20.

Kubota K . Association of serum beta 2-microglobulin with H-2 class I heavy chains on the surface of mouse cells in culture. J Immunol. 1984; 133(6):3203-10. View