Decrease in the Number of DNA Topological Turns During Friend Erythroleukemia Differentiation

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Jan 1

PMID 6930535

Citations 5

Authors

A N Luchnik

V M Glaser

Affiliations

Soon will be listed here.

Abstract

Murine erythroleukemia cells were induced to undergo erythroid differentiation by growing in presence of dimethylsulfoxide, butyric acid or actinomycin D. topological linking numbers in closed loops of nuclear DNA were measured by means of centrifugation of nucleoids containing superhelical DNA in sucrose gradients containing varying concentrations of ethidium bromide. All cells were grown to G1 stage of the cell cycle. It was found that the mean density of the DNA topological linking number decreases from 0.076 turns per 10 nucleotide pairs in non-differentiated cells to 0.062 turns in the cells induced to differentiate. This decrease in topological linking number of DNA loops is quite sufficient for the change in the DNA double helix secondary structure which in turn may be responsible for coordinate switch in transcription of genes which control cellular differentiation (Luchnik, 1980a, b).

Citing Articles

DNA topological linking numbers in malignantly transformed Syrian hamster cells.

Luchnik A, Glaser V Mol Gen Genet. 1981; 183(3):553-6.

PMID: 6950190 DOI: 10.1007/BF00268781.

Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin.

Luchnik A, Bakayev V, ZBARSKY I, Georgiev G EMBO J. 1982; 1(11):1353-8.

PMID: 6327265 PMC: 553216. DOI: 10.1002/j.1460-2075.1982.tb01322.x.

Dimethyl sulfoxide-inducible cytoplasmic factor involved in erythroid differentiation in mouse erythroleukemia (Friend) cells.

Watanabe T, Oishi M Proc Natl Acad Sci U S A. 1987; 84(18):6481-5.

PMID: 3476957 PMC: 299101. DOI: 10.1073/pnas.84.18.6481.

DNAaseI-hypersensitive minichromosomes of SV40 possess an elastic torsional strain in DNA.

Luchnik A, Bakayev V, Yugai A, ZBARSKY I, Georgiev G Nucleic Acids Res. 1985; 13(4):1135-49.

PMID: 2987817 PMC: 341061. DOI: 10.1093/nar/13.4.1135.

Relationship of histone acetylation to DNA topology and transcription.

Krajewski W, Luchnik A Mol Gen Genet. 1991; 230(3):442-8.

PMID: 1662766 DOI: 10.1007/BF00280301.

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