The Effects of Clofibrate Feeding on the Metabolism of Palmitate and Erucate in Isolated Hepatocytes

Overview

Journal Lipids

Specialty Biochemistry

Date 1978 Jul 1

PMID 692296

Citations 12

Authors

R Z Christiansen

H Osmundsen

B Borrebaek

J Bremer

Affiliations

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Abstract

The metabolism of palmitate and erucate has been investigated in hepatocytes isolated from control rats and from rats fed 0.3% clofibrate. Clofibrate increased the oxidation of [1-14C]palmitate 1.5 to 2-fold while the esterification was decreased. At a high concentration of palmitate (1.5 mM), the total rate of fatty acid metabolism was stimulated. Clofibrate stimulated both the oxidation (3.5 to 5-fold) and the esterfication (1.7-fold) of [14-14C]erucate. Erucate undergoes chain-shortening in isolated liver cells. This chain-shortening was stimulated at least 2-fold by clofibrate feedings. The isolated mitochondrial fraction from clofibrate-fed rats showed an increased capacity for oxidation of short-chain acylcarnitines (including acetylcarnitine), while the oxidation of palmitoyl- and erucoylcarnitine showed little change. It is suggested that erucate is shortened by the recently detected beta-oxidation system of peroxisomes.

Citing Articles

Peroxisomal fatty acid oxidation as detected by H2O2 production in intact perfused rat liver.

Foerster E, Fahrenkemper T, Rabe U, Graf P, Sies H Biochem J. 1981; 196(3):705-12.

PMID: 7317011 PMC: 1163089. DOI: 10.1042/bj1960705.

A role for 2,4-enoyl-CoA reductase in mitochondrial beta-oxidation of polyunsaturated fatty acids. Effects of treatment with clofibrate on oxidation of polyunsaturated acylcarnitines by isolated rat liver mitochondria.

Osmundsen H, cervenka J, Bremer J Biochem J. 1982; 208(3):749-57.

PMID: 7165730 PMC: 1154027. DOI: 10.1042/bj2080749.

Metabolism of erucic acid in adipocytes isolated from rat epididymal fat.

Christophersen B, Krogstad T, Norseth J Lipids. 1983; 18(2):137-41.

PMID: 6843302 DOI: 10.1007/BF02536108.

Peroxisomal beta-oxidation from endogenous substrates. Demonstration through H2O2 production in the unanaesthetized mouse.

Van den Branden C, Kerckaert I, Roels F Biochem J. 1984; 218(3):697-702.

PMID: 6372785 PMC: 1153397. DOI: 10.1042/bj2180697.

Effects of high-fat diets on hepatic fatty acid oxidation in the rat. Isolation of rat liver peroxisomes by vertical-rotor centrifugation by using a self-generated, iso-osmotic, Percoll gradient.

Neat C, Thomassen M, Osmundsen H Biochem J. 1981; 196(1):149-59.

PMID: 6272750 PMC: 1162977. DOI: 10.1042/bj1960149.

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