Increase of Hepatic Mitochondria on Administration of Ethyl Alpha-p-chlorophenoxyisobutyrate to the Rat

Overview

Journal Biochem J

Specialty Biochemistry

Date 1970 Mar 1

PMID 5441366

Citations 33

Authors

C K Kurup

H N Aithal

T Ramasarma

Affiliations

Soon will be listed here.

Abstract

1. The antihypercholesterolaemic drug ethyl alpha-p-chlorophenoxyisobutyrate when fed to the rat orally or mixed with the diet increased the content of mitochondria in the liver by 50-100%. Other subcellular fractions did not show any significant change. 2. In oxidative activity, respiratory control and phosphorylating ability no significant difference was observed between the mitochondria isolated from the livers of the drug-treated rats and those from normal animals. 3. In agreement with earlier reports, administration of the drug depressed the concentration of serum cholesterol and increased liver weight and the liver content of ubiquinone. However, the increase of ubiquinone was greater in the nuclear than in the mitochondrial protein.

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References

Osorio C, Walton K, Browne C, West D, Whystock P . The effect of chlorphenoxyisobutyrate ('Atromid-S') on the biliary excretion and distribution of thyroxine in the rat. Biochem Pharmacol. 1965; 14(10):1479-81. DOI: 10.1016/0006-2952(65)90181-4. View

AVOY D, SWYRYD E, Gould R . EFFECTS OF ALPHA-P-CHLOROPHENOXYISOBUTYRYL ETHYL ESTER (CPIB) WITH AND WITHOUT ANDROSTERONE ON CHOLESTEROL BIOSYNTHESIS IN RAT LIVER. J Lipid Res. 1965; 6:369-76. View

Jayaraman J, Cotman C, Mahler H, SHARP C . Biochemical correlates of respiratory deficiency. VII. Glucose repression. Arch Biochem Biophys. 1966; 116(1):224-51. DOI: 10.1016/0003-9861(66)90029-4. View

Krishnaiah K, Joshi V, Ramasarma T . Effect of dietary cholesterol and ubiquinone on isoprene synthesis in rat liver. Arch Biochem Biophys. 1967; 121(1):147-53. DOI: 10.1016/0003-9861(67)90019-7. View

Krishnaiah K, Inamdar A, Ramasarma T . Regulation of steroidogenesis by ubiquinone. Biochem Biophys Res Commun. 1967; 27(4):474-8. DOI: 10.1016/s0006-291x(67)80009-3. View

Phillips W, LAKSHMANAN M, Brien R . Effect of chlorophenoxyisobutyrate on rat liver non-saponifiables. Can J Physiol Pharmacol. 1968; 46(1):81-4. DOI: 10.1139/y68-014. View

WESTERFELD W, RICHERT D, RUEGAMER W . The role of the thyroid hormone in the effect of p-chlorophenoxyisobutyrate in rats. Biochem Pharmacol. 1968; 17(6):1003-16. DOI: 10.1016/0006-2952(68)90359-6. View

Osanai M, Rembold H . [Development-dependent mitochondrial enzyme activities in honey bee castes]. Biochim Biophys Acta. 1968; 162(1):22-31. DOI: 10.1016/0005-2728(68)90210-7. View

RUEGAMER W, Ryan N, RICHERT D, WESTERFELD W . The effects of p-chlorophenoxyisobutyrate on the turnover rate and distribution of thyroid hormone in the rat. Biochem Pharmacol. 1969; 18(3):613-24. DOI: 10.1016/0006-2952(69)90086-0. View

10.

Brunner G, Bygrave F . Microsomal marker enzymes and their limitations in distinguishing the outer membrane of rat liver mitochondria from the microsomes. Eur J Biochem. 1969; 8(4):530-4. DOI: 10.1111/j.1432-1033.1969.tb00558.x. View

11.

Krishnaiah K, Ramasarma T . Effect of alpha-p-chlorophenoxyisobutyrate on the metabolism of isoprenoid compounds in the rat. Biochem J. 1970; 116(3):321-7. PMC: 1185368. DOI: 10.1042/bj1160321. View

12.

HOGEBOOM G, SCHNEIDER W . Cytochemical studies of mammalian tissues. III. Isocitric dehydrogenase and triphosphopyridine nucleotide-cytochrome c reductase of mouse liver. J Biol Chem. 1950; 186(2):417-27. View

13.

Thorp J, Waring W . Modification of metabolism and distribution of lipids by ethyl chlorophenoxyisobutyrate. Nature. 1962; 194:948-9. DOI: 10.1038/194948a0. View

14.

Jayaraman J, Ramasarma T . INTRACELLULAR DISTRIBUTION OF COENZYME Q IN RAT LIVER. Arch Biochem Biophys. 1963; 103:258-66. DOI: 10.1016/0003-9861(63)90403-x. View

15.

Lee M, Whitehouse M . THE EFFECT OF BILE SALTS AND SOME BILE-SALT ANALOGUES ON THE OXIDATION OF CHOLESTEROL BY LIVER MITOCHONDRIA. Biochem J. 1963; 89:189-95. PMC: 1202345. DOI: 10.1042/bj0890189. View

16.

Paget G . EXPERIMENTAL STUDIES OF THE TOXICITY OF ATROMID WITH PARTICULAR REFERENCE TO FINE STRUCTURAL CHANGES IN THE LIVERS OF RODENTS. J Atheroscler Res. 1963; 3:729-36. DOI: 10.1016/s0368-1319(63)80059-9. View

17.

Chance B, Williams G . Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem. 1955; 217(1):409-27. View

18.

SWANSON M . Phosphatases of liver. I. Glucose-6-phosphatase. J Biol Chem. 1950; 184(2):647-59. View

19.

Hess R, Staubli W, RIESS W . Nature of the hepatomegalic effect produced by ethyl-chlorophenoxy-isobutyrate in the rat. Nature. 1965; 208(5013):856-8. DOI: 10.1038/208856a0. View