The Binding Properties of the Muscarinic Receptors of the Cynomolgus Monkey Ciliary Body and the Response to the Induction of Agonist Subsensitivity

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1982 Dec 1

PMID 6897523

Citations 4

Authors

E BARANY

C P Berrie

N J Birdsall

A S BURGEN

E C Hulme

Affiliations

Soon will be listed here.

Abstract

1 The binding properties of the muscarinic receptors in the ciliary muscle of cynomolgus monkeys have been evaluated. 2 The concentration of receptor binding sites is the highest yet reported. As found in many species and tissues, there are subclasses of agonist binding sites. Agonist binding is not affected by the non-hydrolysable guanosine triphosphate (GTP) analogue, GppNHp, suggesting that these receptors are not linked to adenylate cyclase. 3 Ciliary muscles made subsensitive by treatment with muscarinic agonists have a decreased receptor concentration but no other changes in the binding properties of the receptors could be detected.

Citing Articles

Examination of signalling pathways involved in muscarinic responses in bovine ciliary muscle using YM-254890, an inhibitor of the Gq/11 protein.

Yasui F, Miyazu M, Yoshida A, Naruse K, Takai A Br J Pharmacol. 2008; 154(4):890-900.

PMID: 18536740 PMC: 2439854. DOI: 10.1038/bjp.2008.140.

Muscarinic receptor M1 and M2 subtypes in the human eye: QNB, pirenzipine, oxotremorine, and AFDX-116 in vitro autoradiography.

Gupta N, McAllister R, Drance S, Rootman J, Cynader M Br J Ophthalmol. 1994; 78(7):555-9.

PMID: 7918268 PMC: 504863. DOI: 10.1136/bjo.78.7.555.

Muscarinic subsensitivity without receptor change in monkey ciliary muscle.

BARANY E Br J Pharmacol. 1985; 84(1):193-8.

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Measurements of intracellular calcium and contractility in human ciliary muscle.

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PMID: 1945746 DOI: 10.1007/BF00370567.

References

Laties A, JACOBOWITZ D . A comparative study of the autonomic innervation of the eye in monkey, cat, and rabbit. Anat Rec. 1966; 156(4):383-95. DOI: 10.1002/ar.1091560403. View

Van der Zypen E . [Light and electron microscopic studies of the structure and innervation of the ciliary muscle in the human and monkey (Cercopithecus aethiops)]. Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1967; 174(2):143-68. DOI: 10.1007/BF00413512. View

Bito L, Dawson M, Petrinovic L . Cholinergic sensitivity: normal variability as a function of stimulus background. Science. 1971; 172(3983):583-5. DOI: 10.1126/science.172.3983.583. View

BARANY E . Subsensitivity to pilocarpine in primate ciliary muscle following topical anticholinesterase treatment. Invest Ophthalmol. 1975; 14(4):302-6. View

Birdsall N, Hulme E . Biochemical studies on muscarinic acetylcholine receptors. J Neurochem. 1976; 27(1):7-16. DOI: 10.1111/j.1471-4159.1976.tb01536.x. View

BARANY E . Pilocarpine-induced subsensitivity to carbachol and pilocarpine of ciliary muscle in vervet and cynomolgus monkeys. Acta Ophthalmol (Copenh). 1977; 55(1):141-63. DOI: 10.1111/j.1755-3768.1977.tb06103.x. View

Birdsall N, BURGEN A, Hulme E . The binding of agonists to brain muscarinic receptors. Mol Pharmacol. 1978; 14(5):723-36. View

Hulme E, Birdsall N, BURGEN A, Mehta P . The binding of antagonists to brain muscarinic receptors. Mol Pharmacol. 1978; 14(5):737-50. View

Bito L, Baroody R . Gradual changes in the sensitivity of rhesus monkey eyes to miotics and the dependence of these changes on the regimen of topical cholinesterase inhibitor treatment. Invest Ophthalmol Vis Sci. 1979; 18(8):794-801. View

10.

Uchida S, Takeyasu K, Matsuda T, Yoshida H . Changes in muscarinic acetylcholine receptors of mice by chronic administrations of diisopropylfluorophosphate and papaverine. Life Sci. 1979; 24(19):1805-12. DOI: 10.1016/0024-3205(79)90071-7. View

11.

Berrie C, Birdsall N, BURGEN A, Hulme E . Guanine nucleotides modulate muscarinic receptor binding in the heart. Biochem Biophys Res Commun. 1979; 87(4):1000-5. DOI: 10.1016/s0006-291x(79)80006-6. View

12.

Sachs D, Kloog Y, Korczyn A, Heron D, Sokolovsky M . Denervation, supersensitivity and muscarinic receptors in the cat iris. Biochem Pharmacol. 1979; 28(9):1513-8. DOI: 10.1016/0006-2952(79)90466-0. View

13.

Wei J, Sulakhe P . Agonist-antagonist interactions with rat atrial muscarinic cholinergic receptor sites: differential regulation by guanine nucleotides. Eur J Pharmacol. 1979; 58(1):91-2. DOI: 10.1016/0014-2999(79)90346-7. View

14.

Hammer R, Berrie C, Birdsall N, BURGEN A, Hulme E . Pirenzepine distinguishes between different subclasses of muscarinic receptors. Nature. 1980; 283(5742):90-2. DOI: 10.1038/283090a0. View

15.

Rosenberger L, Yamamura H, Roeske W . Cardiac muscarinic cholinergic receptor binding is regulated by Na+ and guanyl nucleotides. J Biol Chem. 1980; 255(3):820-3. View

16.

Shifrin G, Klein W . Regulation of muscarinic acetylcholine receptor concentration in cloned neuroblastoma cells. J Neurochem. 1980; 34(4):993-9. DOI: 10.1111/j.1471-4159.1980.tb09676.x. View

17.

Birdsall N, Hulme E, Burgen A . The character of the muscarinic receptors in different regions of the rat brain. Proc R Soc Lond B Biol Sci. 1980; 207(1166):1-12. DOI: 10.1098/rspb.1980.0011. View

18.

Ehlert F, KOKKA N, Fairhurst A . Altered [3H]quinuclidinyl benzilate binding in the striatum of rats following chronic cholinesterase inhibition with diisopropylfluorophosphate. Mol Pharmacol. 1980; 17(1):24-30. View

19.

Galper J, Smith T . Agonist and guanine nucleotide modulation of muscarinic cholinergic receptors in cultured heart cells. J Biol Chem. 1980; 255(20):9571-9. View

20.

Ehlert F, Roeske W, Yamamura H . Muscarinic receptor: regulation by guanine nucleotides, ions, and N-ethylmaleimide. Fed Proc. 1981; 40(2):153-9. View