Evidence in Support of the P1/P2 Purinoceptor Hypothesis in the Guinea-pig Taenia Coli

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1981 Jul 1

PMID 6894712

Citations 22

Authors

C M Brown

G Burnstock

Affiliations

Soon will be listed here.

Abstract

1 The relaxations induced by adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate (AMP) and adenosine on the carbachol-contracted taenia coli of the guinea-pig have been studied. ATP and ADP produce similar responses which differ in nature and time course from those of AMP and adenosine. 2 Theophylline, at concentrations (25-200 muM) lower than those which produce significant phosphodiesterase inhibition, blocks the effects of AMP and adenosine but fails to antagonize the responses elicited by ATP and ADP. The antagonism of adenosine by theophylline appears to be competitive. 3 Apamin (1-100 nM) blocks the inhibitory effects of ATP and ADP but fails to antagonize the responses to AMP and adenosine. The antagonism by apamin is non-competitive. 4 The results indicate that ATP and adenosine relax the taenia coli by activating different receptors and are consistent with the P1, P2 purinoceptor hypothesis.

Citing Articles

Purinergic signalling in the gastrointestinal tract and related organs in health and disease.

Burnstock G Purinergic Signal. 2013; 10(1):3-50.

PMID: 24307520 PMC: 3944042. DOI: 10.1007/s11302-013-9397-9.

Purine- and pyrimidine-induced responses and P2Y receptor characterization in the hamster proximal urethra.

Pinna C, Glass R, Knight G, Bolego C, Puglisi L, Burnstock G Br J Pharmacol. 2005; 144(4):510-8.

PMID: 15655529 PMC: 1576028. DOI: 10.1038/sj.bjp.0706047.

Kinetics of contraction in depolarized smooth muscle from guinea-pig taenia coli after photodestruction of nifedipine.

Malmqvist U, Arner A J Physiol. 1999; 519 Pt 1:213-21.

PMID: 10432352 PMC: 2269492. DOI: 10.1111/j.1469-7793.1999.0213o.x.

Pharmacological analysis of the interaction between purinoceptor agonists and antagonists in the guinea-pig taenia caecum.

Prentice D, Shankley N, Black J Br J Pharmacol. 1995; 115(4):549-56.

PMID: 7582471 PMC: 1908477. DOI: 10.1111/j.1476-5381.1995.tb14967.x.

The effect of apamin on non-adrenergic, non-cholinergic vasodilator mechanisms in the intestines of the cat.

Jodal M, Lundgren O, Sjoqvist A J Physiol. 1983; 338:207-19.

PMID: 6875956 PMC: 1197190. DOI: 10.1113/jphysiol.1983.sp014669.

References

WENKE M, Lincova D, CEPELIK J, CERNOHORSKY M, Hynie S . Some aspects of the action of beta adrenergic blocking drugs on adrenergic lipid mobilization. Ann N Y Acad Sci. 1967; 139(3):860-78. DOI: 10.1111/j.1749-6632.1967.tb41256.x. View

Isaacson A, Sandow A . Quinine and caffeine effects on 45Ca movements in frog sartorius muscle. J Gen Physiol. 1967; 50(8):2109-28. PMC: 2225767. DOI: 10.1085/jgp.50.8.2109. View

Burnstock G, Campbell G, Satchell D, Smythe A . Evidence that adenosine triphosphate or a related nucleotide is the transmitter substance released by non-adrenergic inhibitory nerves in the gut. Br J Pharmacol. 1970; 40(4):668-88. PMC: 1702901. DOI: 10.1111/j.1476-5381.1970.tb10646.x. View

Schrader J, BERNE R, Rubio R . Uptake and metabolism of adenosine by human erythrocyte ghosts. Am J Physiol. 1972; 223(1):159-66. DOI: 10.1152/ajplegacy.1972.223.1.159. View

Burnstock G, Dumsday B, Smythe A . Atropine resistant excitation of the urinary bladder: the possibility of transmission via nerves releasing a purine nucleotide. Br J Pharmacol. 1972; 44(3):451-61. PMC: 1665813. DOI: 10.1111/j.1476-5381.1972.tb07283.x. View

Samir Amer M, KREIGHBAUM W . Cyclic nucleotide phosphodiesterases: properties, activators, inhibitors, structure--activity relationships, and possible role in drug development. J Pharm Sci. 1975; 64(1):1-37. DOI: 10.1002/jps.2600640106. View

Sjoberg B, Wahlstrom B . The effect of ATP and related compounds on spontaneous mechanical activity in the rat portal vein. Acta Physiol Scand. 1975; 94(1):46-53. DOI: 10.1111/j.1748-1716.1975.tb05860.x. View

Satchell D, Maguire M . Inhibitory effects of adenine nucleotide analogs on the isolated guinea-pig taenia coli. J Pharmacol Exp Ther. 1975; 195(3):540-8. View

Spedding M, WEETMAN D . Identification of separate receptors for adenosine and adenosine 5'-triphosphate in causing relaxations of the isolated taenia of the guinea-pig caecum. Br J Pharmacol. 1976; 57(2):305-10. PMC: 1667108. DOI: 10.1111/j.1476-5381.1976.tb07480.x. View

10.

Burnstock G . Purinergic receptors. J Theor Biol. 1976; 62(2):491-503. DOI: 10.1016/0022-5193(76)90133-8. View

11.

Okwuasaba F, Hamilton J, COOK M . Relaxations of guinea-pig fundic strip by adenosine, adenine nucleotides and electrical stimulation: antagonsism by theophylline and desensitization to adenosine and its derivatives. Eur J Pharmacol. 1977; 46(3):181-98. DOI: 10.1016/0014-2999(77)90333-8. View

12.

Londos C, Wolff J . Two distinct adenosine-sensitive sites on adenylate cyclase. Proc Natl Acad Sci U S A. 1977; 74(12):5482-6. PMC: 431772. DOI: 10.1073/pnas.74.12.5482. View

13.

Vladimirova I, Shuba M . [Effect of strychnine, hydrastine and apamin on synaptic transmission in smooth muscle cells]. Neirofiziologiia. 1978; 10(3):295-9. View

14.

Baidan L, Vladimirova I, Miroshnikov A, Taran G . [Effect of apamin on synaptic transmission in different types of synapses]. Dokl Akad Nauk SSSR. 1978; 241(5):1224-7. View

15.

Fain J, Malbon C . Regulation of adenylate cyclase by adenosine. Mol Cell Biochem. 1979; 25(3):143-69. DOI: 10.1007/BF00235364. View

16.

BANKS B, Brown C, Burgess G, Burnstock G, Claret M, Cocks T . Apamin blocks certain neurotransmitter-induced increases in potassium permeability. Nature. 1979; 282(5737):415-7. DOI: 10.1038/282415a0. View

17.

Braun S, Levitzki A . The attenuation of epinephrine-dependent adenylate cyclase by adenosine and the characteristics of the adenosine stimulatory and inhibitory sites. Mol Pharmacol. 1979; 16(3):737-48. View

18.

Maas A, Den Hertog A . The effect of the phenyl phosphonate N-0164 on prostaglandin action and on post inhibitory excitation in the taenia of guinea-pig caecum. Eur J Pharmacol. 1980; 62(2-3):157-66. DOI: 10.1016/0014-2999(80)90272-1. View

19.

BULBRING E . Measurements of oxygen consumption in smooth muscle. J Physiol. 1953; 122(1):111-34. PMC: 1366182. DOI: 10.1113/jphysiol.1953.sp004983. View

20.

ARUNLAKSHANA O, SCHILD H . Some quantitative uses of drug antagonists. Br J Pharmacol Chemother. 1959; 14(1):48-58. PMC: 1481829. DOI: 10.1111/j.1476-5381.1959.tb00928.x. View