Virulence of Different Pseudomonas Species in a Burned Mouse Model: Tissue Colonization by Pseudomonas Cepacia

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1983 Sep 1

PMID 6885156

Citations 13

Authors

G B Stover

D R Drake

T C MONTIE

Affiliations

Soon will be listed here.

Abstract

The virulence of Pseudomonas aeruginosa and other pseudomonads was examined in a burned mouse model. P. aeruginosa M-2 was highly virulent causing 100% mortality by 38 h with an injection of 10(2) CFU by either a subcutaneous or intraperitoneal route. Subcutaneous injection of 10(2) CFU revealed rapid multiplication of the bacteria at the burn wound with 10(8) CFU/g detectable in the burned skin by 28 h postinjection, 10(5) CFU/g of liver, and 10(3) CFU/ml of blood. Non-P. aeruginosa clinical isolates were markedly less virulent; an injection of greater than or equal to 10(7) CFU caused less than or equal to 60% lethality. P. cepacia SMH colonized the burned skin of thermally injured mice, persisting at levels of 10(7) to 10(8) CFU/g of burned skin after an initial injection of 10(5) CFU. P. cepacia persisted in the burn wound for at least 3 weeks. No organ invasion was detectable throughout this period. Studies with an additional clinical isolate of P. cepacia yielded similar results. An injection of a 10(2) CFU dose revealed that the level of persistence is dose dependent. Results suggest that the tenacious persistence of P. cepacia in the burn wound may provide a model for the study of persistent colonization and infection in a compromised host.

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